食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
20期
149-151
,共3页
芸香苷%异槲皮苷%黄瓜籽%HPLC%双波长%含量测定
蕓香苷%異槲皮苷%黃瓜籽%HPLC%雙波長%含量測定
예향감%이곡피감%황과자%HPLC%쌍파장%함량측정
rutin%isoquercitrin%cucumber seeds%HPLC%double-wavelength%content determination
建立HPLC双波长法同时测定黄瓜籽中芸香苷、异槲皮苷含量的方法。以shim-pack VP-ODS C18（250 mm×4.6 mm，5μm）为固定相，以乙腈-0.2%磷酸溶液（15∶85）为流动相，流速1.0 mL/min，检测波长为254、273 nm，柱温30℃。芸香苷、异槲皮苷分别在0.00159 mg/mL～0.159 mg/mL（r=0.9998）和0.00106 mg/mL～0.106 mg/mL（r=0.9995）线性范围内与峰面积呈良好的线性关系，平均回收率分别为99.24%（RSD=0.4%，n=6）和98.56%（RSD=0.5%，n=6）。该方法快速、简便、准确、可靠。
建立HPLC雙波長法同時測定黃瓜籽中蕓香苷、異槲皮苷含量的方法。以shim-pack VP-ODS C18（250 mm×4.6 mm，5μm）為固定相，以乙腈-0.2%燐痠溶液（15∶85）為流動相，流速1.0 mL/min，檢測波長為254、273 nm，柱溫30℃。蕓香苷、異槲皮苷分彆在0.00159 mg/mL～0.159 mg/mL（r=0.9998）和0.00106 mg/mL～0.106 mg/mL（r=0.9995）線性範圍內與峰麵積呈良好的線性關繫，平均迴收率分彆為99.24%（RSD=0.4%，n=6）和98.56%（RSD=0.5%，n=6）。該方法快速、簡便、準確、可靠。
건립HPLC쌍파장법동시측정황과자중예향감、이곡피감함량적방법。이shim-pack VP-ODS C18（250 mm×4.6 mm，5μm）위고정상，이을정-0.2%린산용액（15∶85）위류동상，류속1.0 mL/min，검측파장위254、273 nm，주온30℃。예향감、이곡피감분별재0.00159 mg/mL～0.159 mg/mL（r=0.9998）화0.00106 mg/mL～0.106 mg/mL（r=0.9995）선성범위내여봉면적정량호적선성관계，평균회수솔분별위99.24%（RSD=0.4%，n=6）화98.56%（RSD=0.5%，n=6）。해방법쾌속、간편、준학、가고。
To set up an HPLC method of double-wavelength simultaneous determination of the content for rutin and isoquercitrin in Cucumber seeds. The solid phase was shim-pack VP-DOS C18 (250 mm×4.6 mm,5μm). The mobile phase was composed of acetonitrile-0.2%Phosphoric acid (15∶85), the flow rate was 1.0 mL/min, the detection wavelength was set at 254 nm and 273 nm ,the temperature of chromatographic column was 30℃. good linear relationships were found within the range of 0.001 59 mg/mL-0.159 mg/mL (r=0.999 8)of rutin and 0.001 06 mg/mL-0.106 mg/mL (r=0.999 5) of isoquercitrin the average of recovery rate was 99.24%(RSD=0.4%,n=6) and 98.56%(RSD=0.5%,n=6). The results of the study showed that this method was rapid, simple, accurate and reliable.
