西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
Journal of Xi'an Jiaotong University (Medical Sciences)
2015年
6期
787-791
,共5页
李云肖%殷芳圆%乔晓孟%魏曙光%赖江华
李雲肖%慇芳圓%喬曉孟%魏曙光%賴江華
리운초%은방원%교효맹%위서광%뢰강화
脑源性神经营养因子(BDNF)%海洛因依赖%单核苷酸多态性
腦源性神經營養因子(BDNF)%海洛因依賴%單覈苷痠多態性
뇌원성신경영양인자(BDNF)%해락인의뢰%단핵감산다태성
brain-derived neurotrophic factor (BDNF)%heroin dependence%single nucleotide polymorphism
目的：探讨脑源性神经营养因子(BDNF)基因多态性与海洛因依赖的相关性。方法对符合纳入及排除标准的海洛因依赖组308例及健康对照组317例进行临床资料收集与血样采集。采用飞行时间质谱分析方法对 BDNF rs16917204、rs6265、rs7103873、rs7103873、rs56164415、rs13306221、rs2030323共7个多态性位点进行分型,数据采用SPSS 20.0和 HaploView 4.0软件进行统计学分析。结果rs6265位点基因型频率分布在海洛因依赖组及健康对照组中存在统计学差异(χ2=15.151,P =0.001)。与健康对照组相比,海洛因依赖组 rs6265位点 G 等位基因频率显著高于健康对照组(χ2=9.864,P =0.002,OR=1.429,95% CI =1.143~1.786)。rs13306221位点基因型频率分布在海洛因依赖组及健康对照组中存在统计学差异(χ2=7.699,P =0.006)。与健康对照组相比,海洛因依赖组 G等位基因频率显著高于健康对照组(χ2=7.137,P =0.008,OR=0.539,95% CI =0.340~0.853)。连锁不平衡检验发现,由 rs6265与 rs71038731(block1)构建的单倍型为强连锁不平衡(D’>0.9；r 2>0.8)。在海洛因依赖组及健康对照组中,G-G 单倍型频率分布存在显著性差异,健康对照组中 G-G 单倍型频率显著高于海洛因依赖组(χ2=4.546,P =0.033)。结论BDNF 基因 rs6265及 rs13306221多态性位点可能与海洛因依赖有关,携带有 rs6265位点G 等位基因及 rs13306221位点 G 等位基因的个体更易形成海洛因依赖,携带有 G-G 单倍型(rs6265-rs71038731)的个体相对不易对海洛因产生依赖。
目的：探討腦源性神經營養因子(BDNF)基因多態性與海洛因依賴的相關性。方法對符閤納入及排除標準的海洛因依賴組308例及健康對照組317例進行臨床資料收集與血樣採集。採用飛行時間質譜分析方法對 BDNF rs16917204、rs6265、rs7103873、rs7103873、rs56164415、rs13306221、rs2030323共7箇多態性位點進行分型,數據採用SPSS 20.0和 HaploView 4.0軟件進行統計學分析。結果rs6265位點基因型頻率分佈在海洛因依賴組及健康對照組中存在統計學差異(χ2=15.151,P =0.001)。與健康對照組相比,海洛因依賴組 rs6265位點 G 等位基因頻率顯著高于健康對照組(χ2=9.864,P =0.002,OR=1.429,95% CI =1.143~1.786)。rs13306221位點基因型頻率分佈在海洛因依賴組及健康對照組中存在統計學差異(χ2=7.699,P =0.006)。與健康對照組相比,海洛因依賴組 G等位基因頻率顯著高于健康對照組(χ2=7.137,P =0.008,OR=0.539,95% CI =0.340~0.853)。連鎖不平衡檢驗髮現,由 rs6265與 rs71038731(block1)構建的單倍型為彊連鎖不平衡(D’>0.9；r 2>0.8)。在海洛因依賴組及健康對照組中,G-G 單倍型頻率分佈存在顯著性差異,健康對照組中 G-G 單倍型頻率顯著高于海洛因依賴組(χ2=4.546,P =0.033)。結論BDNF 基因 rs6265及 rs13306221多態性位點可能與海洛因依賴有關,攜帶有 rs6265位點G 等位基因及 rs13306221位點 G 等位基因的箇體更易形成海洛因依賴,攜帶有 G-G 單倍型(rs6265-rs71038731)的箇體相對不易對海洛因產生依賴。
목적：탐토뇌원성신경영양인자(BDNF)기인다태성여해락인의뢰적상관성。방법대부합납입급배제표준적해락인의뢰조308례급건강대조조317례진행림상자료수집여혈양채집。채용비행시간질보분석방법대 BDNF rs16917204、rs6265、rs7103873、rs7103873、rs56164415、rs13306221、rs2030323공7개다태성위점진행분형,수거채용SPSS 20.0화 HaploView 4.0연건진행통계학분석。결과rs6265위점기인형빈솔분포재해락인의뢰조급건강대조조중존재통계학차이(χ2=15.151,P =0.001)。여건강대조조상비,해락인의뢰조 rs6265위점 G 등위기인빈솔현저고우건강대조조(χ2=9.864,P =0.002,OR=1.429,95% CI =1.143~1.786)。rs13306221위점기인형빈솔분포재해락인의뢰조급건강대조조중존재통계학차이(χ2=7.699,P =0.006)。여건강대조조상비,해락인의뢰조 G등위기인빈솔현저고우건강대조조(χ2=7.137,P =0.008,OR=0.539,95% CI =0.340~0.853)。련쇄불평형검험발현,유 rs6265여 rs71038731(block1)구건적단배형위강련쇄불평형(D’>0.9；r 2>0.8)。재해락인의뢰조급건강대조조중,G-G 단배형빈솔분포존재현저성차이,건강대조조중 G-G 단배형빈솔현저고우해락인의뢰조(χ2=4.546,P =0.033)。결론BDNF 기인 rs6265급 rs13306221다태성위점가능여해락인의뢰유관,휴대유 rs6265위점G 등위기인급 rs13306221위점 G 등위기인적개체경역형성해락인의뢰,휴대유 G-G 단배형(rs6265-rs71038731)적개체상대불역대해락인산생의뢰。
Objective To examine the association between the polymorphisms of brain-derived neurotrophic factor (BDNF)gene with heroin dependence.Methods Genomic DNA was isolated from the venous blood leukocytes of 308 unrelated patients with heroin dependence and 31 7 healthy individuals.Seven single nucleotide polymorphisms (SNPs)were genotyped using MassARRAY system.Data were analyzed using HaploView 4.0 and SPSS 20.0 software.Results There was a significant difference in the genotype frequency of rs6265 between heroin dependence group and healthy control group (χ2 =1 5.1 5 1,P =0.001).The rs6265 G allele was significantly higher than in controls (χ2 =9.864,P =0.002,OR =1.429,95% CI =1.143 -1.786).Furthermore,there was also a significant difference in the genotype frequency of rs13306221 between heroin dependence group and control group (χ2 =7.699,P =0.006).The rs13306221 G allele was significantly higher than in controls (χ2 =7.137,P =0.008,OR =0.539,95% CI =0.340-0.853).Strong linkage disequilibrium (LD)was observed in one block (D’> 0.9;r 2 >0.8),and significantly less G-G haplotype frequency of block 1 (χ2 =4.546;P =0.033)was found in heroin dependence group. Conclusion Our findings support the role of BDNF rs6265 and rs13306221 polymorphisms in heroin dependence and may guide future studies to identify other genetic risk factors for heroin dependence.
