CAJ | 학술논문

研究连续提取并分离葡萄籽中的葡萄籽油和多酚的最佳工艺条件。将干燥的葡萄籽粉末用石油醚湿润装进层析柱中，浸泡2 h，先后用石油醚和60%乙醇溶液连续洗脱，连续收集石油醚6倍体积（mL/g）和60%乙醇溶液10倍体积洗脱液，混合洗脱液分层，醚层中含有葡萄籽油，下层醇水液中含有多酚。连续提取的葡萄籽油和多酚的提取率均达到99%，经萃取一次性分离葡萄籽油和多酚，得率分别是97.6%和98.0%。利用柱层析连续提取葡萄籽油和多酚，并通过萃取可把两种成分一次性分离开来，工艺简单，时间短，有机溶剂用量少，提取效率高，具有广泛应用价值。
연구련속제취병분리포도자중적포도자유화다분적최가공예조건。장간조적포도자분말용석유미습윤장진층석주중，침포2 h，선후용석유미화60%을순용액련속세탈，련속수집석유미6배체적（mL/g）화60%을순용액10배체적세탈액，혼합세탈액분층，미층중함유포도자유，하층순수액중함유다분。련속제취적포도자유화다분적제취솔균체도99%，경췌취일차성분리포도자유화다분，득솔분별시97.6%화98.0%。이용주층석련속제취포도자유화다분，병통과췌취가파량충성분일차성분리개래，공예간단，시간단，유궤용제용량소，제취효솔고，구유엄범응용개치。
To explore technical process and conditions for the sequential extraction of grape seed oil and polyphenol by using column chromatography from grape seed. Dried material was loaded into a column with petroleum ether and sequentially eluted with 6-fold (mL/g) petroleum ether, 10-fold 60% (v/v) ethanol. The elutes was separated into an ether fraction containing grape seed oil and an ethanol-water fraction containing polyphenol. Through this procedure, grape seed oil and polyphenol in grape seed were simultaneously extracted at 99%extraction rates and simply separated at higher than 97.6%and 98.0%recovery rates. The process was simple and short, with less consumption of solvents and high extraction rates. The method provides a simple and high-efficient extraction and separation of wide range bioactive substances.