CAJ | 학술논문

目的：研究转化生长因子β1(TGF-β1)对牙周膜干细胞(PDLSCs)的迁移、粘附和增殖的影响,初步探讨 TGF-β1通过影响牙周组织干细胞参与牙周改建的机制。方法分离培养人 PDLSCs,鉴定其多向分化潜能。用 Transwell 法检测 TGF-β1对 PDLSCs 的迁移的影响。用粘附实验检测 TGF-β1对 PDLSCs 粘附能力的影响。用 MTT 法和生长率法检测 TGF-β1对 PDLSCs 增殖的影响。结果浓度为2.5 ng/mL 以及10 ng/mL 的 TGF-β1可促进 PDLSCs 的迁移,而且此作用呈剂量效应关系。粘附实验结果显示,在分别作用2 h 和4 h 后,浓度为10 ng/mL 的 TGF-β1可促进PDLSCs 的粘附。MTT 法的结果证实了 TGF-β1可促进 PDLSCs 的增殖,该作用呈剂量效应关系。再将细胞在含有或者不含有10 ng/mLTGF-β1的培养基中培养2、4、6 d,对细胞进行计数后发现 TGF-β1显著促进 PDLSCs 的生长。结论TGF-β1可促进 PDLSCs 的迁移、粘附和增殖。推测 TGF-β1可能通过诱导 PDLSCs 向牙周组织部位迁移并提高其粘附和增殖能力。
목적：연구전화생장인자β1(TGF-β1)대아주막간세포(PDLSCs)적천이、점부화증식적영향,초보탐토 TGF-β1통과영향아주조직간세포삼여아주개건적궤제。방법분리배양인 PDLSCs,감정기다향분화잠능。용 Transwell 법검측 TGF-β1대 PDLSCs 적천이적영향。용점부실험검측 TGF-β1대 PDLSCs 점부능력적영향。용 MTT 법화생장솔법검측 TGF-β1대 PDLSCs 증식적영향。결과농도위2.5 ng/mL 이급10 ng/mL 적 TGF-β1가촉진 PDLSCs 적천이,이차차작용정제량효응관계。점부실험결과현시,재분별작용2 h 화4 h 후,농도위10 ng/mL 적 TGF-β1가촉진PDLSCs 적점부。MTT 법적결과증실료 TGF-β1가촉진 PDLSCs 적증식,해작용정제량효응관계。재장세포재함유혹자불함유10 ng/mLTGF-β1적배양기중배양2、4、6 d,대세포진행계수후발현 TGF-β1현저촉진 PDLSCs 적생장。결론TGF-β1가촉진 PDLSCs 적천이、점부화증식。추측 TGF-β1가능통과유도 PDLSCs 향아주조직부위천이병제고기점부화증식능력。
Objective To evaluate the effects of transforming growth factor β1 (TGF-β1 )on migration, adhesion and proliferation of periodontal ligament stem cells (PDLSCs)and explore the mechanisms of PDLSCs-induced periodontal remodeling.Methods PDLSCs were isolated and identified from human teeth.The effect of TGF-β1 on migration of PDLSCs was evaluated using transwell migration assay.Cells attachment assay was used to test the effect of TGF-β1 on the adhesion of PDLSCs.In addition,the effect of TGF-β1 on the proliferation of PDLSCs was evaluated by MTT and cell growth rate assay.Results The results showed that TGF-β1 induced the migration of PDLSCs in a dose-dependent manner,improved the adhesion and proliferation of PDLSCs.So we propose that TGF-β1 may promote periodium remodeling by inducing PDLSCs migration,following adhesion and proliferation in these areas.Conclusion This study demonstrated for the first time that TGF-β1 increases the adhesion and migration of PDLSCs in vitro .The signal pathway is involved in the TGF-β1-induced migration of PDLSCs and the mechanical-chemical interaction during the orthodontic periodontal remodeling will be researched in our further studies.