CAJ | 학술논문

目的：探讨脂肪酸合成酶(fatty acid synthase,FASN)对胰腺癌细胞凋亡的影响及其可能的分子机制。方法应用 Annexin V/FITC 流式细胞仪检测脂肪酸合成酶受到抑制后对胰腺癌细胞株 PANC-1凋亡水平的影响,qRT-PCR、Western blot 技术检测受到 C75作用后的胰腺癌细胞 PANC-1中 Caspase-3、Bcl-2、FASN 蛋白及 RNA 的水平。结果应用 C75对 PANC-1细胞中 FASN 进行特异性地抑制,随着 C75浓度增加,胰腺癌细胞中脂肪酸合成酶蛋白表达量明显降低,细胞凋亡水平升高。同时,C75处理组 Caspase-3分子水平较对照组升高,Bcl-2分子水平下降(P <0.05)。结论脂肪酸合成酶通过 Bcl-2以及 Caspase-3分子,参与了胰腺癌细胞株 PANC-1中细胞凋亡的过程。因此,脂肪酸合成酶在未来可以作为药物靶点,用于临床治疗胰腺癌。
목적：탐토지방산합성매(fatty acid synthase,FASN)대이선암세포조망적영향급기가능적분자궤제。방법응용 Annexin V/FITC 류식세포의검측지방산합성매수도억제후대이선암세포주 PANC-1조망수평적영향,qRT-PCR、Western blot 기술검측수도 C75작용후적이선암세포 PANC-1중 Caspase-3、Bcl-2、FASN 단백급 RNA 적수평。결과응용 C75대 PANC-1세포중 FASN 진행특이성지억제,수착 C75농도증가,이선암세포중지방산합성매단백표체량명현강저,세포조망수평승고。동시,C75처리조 Caspase-3분자수평교대조조승고,Bcl-2분자수평하강(P <0.05)。결론지방산합성매통과 Bcl-2이급 Caspase-3분자,삼여료이선암세포주 PANC-1중세포조망적과정。인차,지방산합성매재미래가이작위약물파점,용우림상치료이선암。
Objective To investigate the effect of fatty acid synthase (FASN)on apoptosis in pancreatic cancer cell PANC-1 and the possible molecular mechanism.Methods Annexin V/FITC and flow cytometry were performed to detect the expression of FASN in pancreatic cancer PANC-1 after C75 treatment and the change of apoptosis in pancreatic cancer cell PANC-1 treated with C75.Quantity reverse transcriptase polymerase chain reaction (RT-PCR)and Western blot were used to measure the protein and RNA expressions of Caspase-3,bcl-2 and FASN.Results Inhibited by C75,the activity of FASN in pancreatic cancer cell PANC-1 was significantly decreased.Meanwhile,PANC-1 showed an increased apoptosis level in a dose-dependent manner (P < 0.05 ). Furthermore,after C75 inhibited FASN in pancreatic cancer cells,the protein and RNA expressions of Caspase-3 significantly increased (P <0.05)whereas the level of Bcl-2 reduced (P <0.05).Conclusion FASN is involved in the process of apoptosis in PANC-1 via Bcl-2 and Caspase-3.Therefore,FASN will provide a new target for the treatment of pancreatic cancer and generate better treatment efficacy.