CAJ | 학술논문

利用HPLC结合MALDI-TOF-MS对1-苯基-3-甲基-5-吡唑啉酮( PMP)衍生化后山毛榉木聚糖水解产物进行分析，检测到了难以获得标准品对照的木聚糖水解产物。结果表明，稀硫酸水解山毛榉木聚糖的主要水解产物有木糖和4-O-甲基葡萄糖醛酸-木糖(B2)，以及少量4-O-甲基葡萄糖醛酸(B1)。内切重组木聚糖酶AnXyn10C水解山毛榉木聚糖产生木糖、木二糖和4-O-甲基葡萄糖醛酸-木三糖(B3)，而内切重组木聚糖酶HoXyn11A水解山毛榉木聚糖主要产生木糖、木二糖、木三糖、4-O-甲基葡萄糖醛酸-木四糖( B4)和4-O-甲基葡萄糖醛酸-木五糖( B5)。基于PMP柱前衍生化的HPLC结合MALDI-TOF-MS方法能高效地分析复杂的木聚糖水解产物。
이용HPLC결합MALDI-TOF-MS대1-분기-3-갑기-5-필서람동( PMP)연생화후산모거목취당수해산물진행분석，검측도료난이획득표준품대조적목취당수해산물。결과표명，희류산수해산모거목취당적주요수해산물유목당화4-O-갑기포도당철산-목당(B2)，이급소량4-O-갑기포도당철산(B1)。내절중조목취당매AnXyn10C수해산모거목취당산생목당、목이당화4-O-갑기포도당철산-목삼당(B3)，이내절중조목취당매HoXyn11A수해산모거목취당주요산생목당、목이당、목삼당、4-O-갑기포도당철산-목사당( B4)화4-O-갑기포도당철산-목오당( B5)。기우PMP주전연생화적HPLC결합MALDI-TOF-MS방법능고효지분석복잡적목취당수해산물。
The hydrolysis end products of beechwood xylan, which were released by sulfuric acid or enzymes and then labeled at their reducing ends with 1-phenyl-3-methyl-5-pyrazolone ( PMP) derivatization, were analyzed by HPLC assisted with MALDI-TOF-MS. Some of the hydrolysates, which were lack of related commercial available standard substances, were determined. It was found that the xylose and 4-O-methyl-glucuronic acid-xylose ( B2 ) were the main products with minor amounts of 4-O-methylglucuronic acid ( B1 ) in the hydrolysates of beechwood xylan by sulfuric acid. Recombinant endo-β-1, 4-xylanase AnXyn10C released xylose, xylobiose, and 4-O-methyl-glucuronic acid-xylotriose (B3) as the main hydrolysates from beechwood xylan, whereas recombinant endo-β-1,4-xylanase HoXyn11A released xylose, xylobiose, xylotriose, 4-O-methyl-glucuronic acid-xylotetrose ( B4 ) and -xylopentaose ( B5 ) , and aldohexaouronic acid. These results revealed that HPLC assisted with MALDI-TOF-MS based on PMP derivatization was a very useful and robust method for the determination of products in hydrolysis of xylan.