CAJ | 학술논문

分别采用复合纤维素酶Celluclast 1.5L和内切纤维素酶Novozym 476对漂白针叶木纤维进行水解,通过分析酶水解后纤维素聚合度和结晶度的变化情况,研究2种纤维素酶对纤维素聚合度和聚集态结构的影响。结果显示：漂白针叶木纤维经2种纤维素酶分别水解后,随着酶用量的增加,纤维素聚合度都逐渐降低,当Celluclast 1.5 L用量为3.0 FPU/g时,纤维得率为94.34%,纤维素聚合度降至862,较原样(1164)下降了25.95%；而Novozym 476用量为50.0 U/g时,纤维得率为94.93%,纤维素聚合度降至711,较原样下降了38.92%。由此可见,在得率相同的情况下,经Novozym 476水解的纤维素较经Celluclast 1.5 L水解的纤维素具有更低的聚合度,说明内切葡聚糖酶是导致纤维素聚合度下降的主要因素。随着复合纤维素酶Celluclast 1.5 L用量的增加,纤维素结晶度呈现先增加后降低再增加再降低的M型变化；随着内切纤维素酶Novozym 476用量的增加,纤维素结晶度呈现先降低后增加再降低的变化。
분별채용복합섬유소매Celluclast 1.5L화내절섬유소매Novozym 476대표백침협목섬유진행수해,통과분석매수해후섬유소취합도화결정도적변화정황,연구2충섬유소매대섬유소취합도화취집태결구적영향。결과현시：표백침협목섬유경2충섬유소매분별수해후,수착매용량적증가,섬유소취합도도축점강저,당Celluclast 1.5 L용량위3.0 FPU/g시,섬유득솔위94.34%,섬유소취합도강지862,교원양(1164)하강료25.95%；이Novozym 476용량위50.0 U/g시,섬유득솔위94.93%,섬유소취합도강지711,교원양하강료38.92%。유차가견,재득솔상동적정황하,경Novozym 476수해적섬유소교경Celluclast 1.5 L수해적섬유소구유경저적취합도,설명내절포취당매시도치섬유소취합도하강적주요인소。수착복합섬유소매Celluclast 1.5 L용량적증가,섬유소결정도정현선증가후강저재증가재강저적M형변화；수착내절섬유소매Novozym 476용량적증가,섬유소결정도정현선강저후증가재강저적변화。
Complex cellulase named Celluclast 1. 5L and endo-cellulase named Novozym 476 were applied to hydrolyze the bleached softwood fiber. In the present study, the influences of these two kinds of cellulases on the degree of polymerization ( DP) and crystallinity of cellulose were investigated. The results indicated that the DP of cellulose decreased gradually with the increase of the enzyme dosage after hydrolyzed by both cellulases. While the dosage of Celluclast 1. 5 L was 3. 0 FPU/g, the yield of cellulose was 94. 34%. The DP of cellulose decreased to 862 and decreased by 25. 95% compared with the control sample (1 164). While the Novozym 476 was in the dosage of 50. 0 U/g, the cellulose yield was 94. 93%. The DP of cellulose decreased to 711 and decreased by 38. 92% compared with the control sample. This showed that in the circumstances of same yield, the cellulose hydrolyzed by Novozym 476 had a lower DP compared with the cellulose hydrolyzed by Celluclast 1. 5 L. It meant that endoglucanase was the main factor which led to the decline of DP. With the increase of enzyme dosage of Celluclast 1. 5 L, cellulose crystallinity took the changes of M-type, in other words, increased first and then reduced, and finally increased and reduced again. With the increase of enzyme dosage of Novozym 476, the cellulose crystallinity decreased first, and then increased and decreased again.