CAJ | 학술논문

研究红叶李叶的化学成分和抗氧化活性。以硅胶、葡聚糖凝胶LH-20、高效制备液相色谱等分离红叶李叶乙醇提取物,根据光谱数据结合理化性质鉴定17个化合物,分别为β-谷甾醇(1)、豆甾醇(2)、胡萝卜苷(3)、羽扇豆醇(4)、熊果酸(5)、阿江榄仁酸(6)、Niga-ichigoside F1(7)、芹菜素(8)、山奈酚(9)、山柰酚-3-O-β-D-葡萄糖苷(10)、槲皮素(11)、槲皮素4′-O-β-D-葡萄糖苷(12)、金丝桃苷(13)、木犀草素(14)、木犀草素-4′-O-β-D-葡萄糖苷(15)、芦丁(16)、没食子酸(17)。所有化合物均为首次从红叶李叶中分离得到。以DPPH自由基清除能力检测所得化合物的抗氧化能力,结果显示,化合物11,14和17表现出显著的自由基清除能力, IC50分别为32.18、34.65和34.12 mmol/L,活性强于Vc(IC50为55.22 mmol/L)。
연구홍협리협적화학성분화항양화활성。이규효、포취당응효LH-20、고효제비액상색보등분리홍협리협을순제취물,근거광보수거결합이화성질감정17개화합물,분별위β-곡치순(1)、두치순(2)、호라복감(3)、우선두순(4)、웅과산(5)、아강람인산(6)、Niga-ichigoside F1(7)、근채소(8)、산내분(9)、산내분-3-O-β-D-포도당감(10)、곡피소(11)、곡피소4′-O-β-D-포도당감(12)、금사도감(13)、목서초소(14)、목서초소-4′-O-β-D-포도당감(15)、호정(16)、몰식자산(17)。소유화합물균위수차종홍협리협중분리득도。이DPPH자유기청제능력검측소득화합물적항양화능력,결과현시,화합물11,14화17표현출현저적자유기청제능력, IC50분별위32.18、34.65화34.12 mmol/L,활성강우Vc(IC50위55.22 mmol/L)。
Ethanol extracts from the leaves of Prunus cerasifera Ehrh. cv. Atropurpurea Jacg. were separated and purified by column chromatography over silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by spectral methods including 1 H, 13C NMR. Seventeen compounds were isolated and identified as β-sitosterol ( 1 ) , stigmasterol ( 2 ) , daucosterol (3), lupeol (4), ursolic acid (5), arjunolic acid (6), Niga-ichigoside F1 (7), apigenin (8), kaempferol (9), kaempferol-3-O-β-D-glucoside (10), quercetin (11), quercetin-4′-O-β-D- glucoside (12), hyperin (13), luteolin (14), luteolin-4′-O-β-D-glucoside (15), rutin (16), gallic acid (17). All compounds were isolated from the leaf for the first time. Compounds 11, 14 and 17 showed stronger antioxidant activities by DPPH scavenging activity with IC50 inhibition rates of 32. 18, 34. 65 and 34. 12 mmol/L, respectively, compared with the Vc IC50 inhibition rate of 55. 22 mmol/L.