CAJ | 학술논문

目的：考察不同提取方法对土茯苓多糖得率及抗氧化活性的影响。方法采用冷水、热水、氢氧化钠溶液、乙二胺四乙酸二钠（EDTA?2Na）水溶液提取土茯苓多糖，测定清除羟自由基活性、对超氧阴离子自由基的清除作用、还原能力，评价土茯苓多糖的体外抗氧化能力。结果冷水溶粗多糖、热水溶粗多糖、碱溶粗多糖、酸溶粗多糖的多糖得率分别为0.31%、1.1%、10.8%、2.0%。4种方法提取所得土茯苓粗多糖清除超氧自由基的能力均较好；冷水溶粗多糖、热水溶粗多糖、酸溶粗多糖清除羟自由基的能力及还原能力较好，且随浓度增加而上升；碱溶粗多糖基本无清除羟自由基的能力及还原能力。结论4种方法提取所得粗多糖均具有一定的抗氧化活性，且各组分的体外抗氧化活性与多糖含量呈正相关性，结合得率，EDTA?2Na 水溶液提取法最好。
목적：고찰불동제취방법대토복령다당득솔급항양화활성적영향。방법채용랭수、열수、경양화납용액、을이알사을산이납（EDTA?2Na）수용액제취토복령다당，측정청제간자유기활성、대초양음리자자유기적청제작용、환원능력，평개토복령다당적체외항양화능력。결과랭수용조다당、열수용조다당、감용조다당、산용조다당적다당득솔분별위0.31%、1.1%、10.8%、2.0%。4충방법제취소득토복령조다당청제초양자유기적능력균교호；랭수용조다당、열수용조다당、산용조다당청제간자유기적능력급환원능력교호，차수농도증가이상승；감용조다당기본무청제간자유기적능력급환원능력。결론4충방법제취소득조다당균구유일정적항양화활성，차각조분적체외항양화활성여다당함량정정상관성，결합득솔，EDTA?2Na 수용액제취법최호。
Objective To explore the effects of different extraction methods on yield of Smilax glabra polysaccharides and antioxidant activities. Methods Crude polysaccharides were extracted by using cold water, hot water, NaOH aqueous solution, and EDTA?2Na aqueous solution . Antioxidant activities of Smilax glabra polysaccharides were compared by the methods of scavenging superoxide anion radical , scavenging hydroxyl radical or reducing power. Results Yield of crude cold water-soluble polysaccharides, crude boiling water-soluble polysaccharides, crude alkali-soluble polysaccharides and crude acid-soluble polysaccharides were 0.31%, 1.1%, 10.8 %, 2.0%, respectively. Polysaccharides by using four kinds of different extraction methods had strong scavenging superoxide anion radical power; crude cold water-soluble polysaccharides, crude boiling water-soluble polysaccharides , crude acid-soluble polysaccharides had strong scavenging hydroxyl radical and reducing power , the capacity increased with the increasing concentration of Smilax glabra polysaccharides; the scavenging hydroxyl radical and reducing power capacity of crude alkali-soluble polysaccharides is very poor. Conclusion Polysaccharides by using four kinds of different extraction methods have certain antioxidant activity, the antioxidant capacity to scavenge free radical in vitro showed positive correlation with the concentration of Smilax glabra polysaccharides, considering the yield of polysaccharides, EDTA?2Na aqueous solution extraction method is the best.