CAJ | 학술논문

目的：观察加味黄芪建中汤对乙酸致慢性胃溃疡模型大鼠溃疡愈合质量的影响，探讨其作用机制。方法将40只 wistar 大鼠按随机数字表法分为正常组、模型组、加味黄芪建中汤组、奥美拉唑组，每组10只，采用100%乙酸诱导慢性胃溃疡大鼠模型。造模后3 d 开始给药，加味黄芪建中汤组灌胃10%加味黄芪建中汤20 ml/kg；奥美拉唑组灌胃3%奥美拉唑混悬液20 ml/kg；正常组、模型组灌胃等体积生理盐水，1次/d。连续给药16 d 后，观察大鼠胃黏膜病理改变、溃疡指数、再生黏膜厚度、黏膜表面黏液厚度、黏膜肌层缺损宽度、囊状扩张腺体数，并检测血清6-酮前列腺素 F1α(6-K-PGF1α)、表皮生长因子(EGF)、一氧化氮(NO)水平。结果与模型组比较，加味黄芪建中汤组大鼠溃疡指数[(8.95±2.78)mm2比(20.82±5.12)mm2]、黏膜肌层缺损宽度[(123.56±32.89)μm 比(229.32±49.69)μm]、囊状腺体扩张数[(1.65±0.76)个比(6.12±1.23)个]降低(P＜0.01)；再生黏膜厚度[(329.55±32.22)μm 比(198.22±5.83)μm]、表面黏液厚度[(44.3±2.8)μm 比(24.5±7.8)μm]、血清6-K-PGF1α[(93.7±8.9)pg/ml比(58.5±5.8)pg/ml]、EGF[(2.11±0.29)ng/L 比(0.82±0.20)ng/L]、NO[(0.51±0.03)μmol/L 比(0.22±0.05)μmol/L]水平升高(P＜0.01)；除溃疡指数外，其他各项指标改善情况加味黄芪建中汤组均优于奥美拉唑组(P＜0.05)。结论加味黄芪建中汤可降低溃疡指数、黏膜肌层缺损宽度及囊状腺体扩张数，提高再生黏膜厚度、黏膜表面黏液厚度及6-K-PGF1α、EGF、NO 水平，提高实验性胃溃疡愈合质量。
목적：관찰가미황기건중탕대을산치만성위궤양모형대서궤양유합질량적영향，탐토기작용궤제。방법장40지 wistar 대서안수궤수자표법분위정상조、모형조、가미황기건중탕조、오미랍서조，매조10지，채용100%을산유도만성위궤양대서모형。조모후3 d 개시급약，가미황기건중탕조관위10%가미황기건중탕20 ml/kg；오미랍서조관위3%오미랍서혼현액20 ml/kg；정상조、모형조관위등체적생리염수，1차/d。련속급약16 d 후，관찰대서위점막병리개변、궤양지수、재생점막후도、점막표면점액후도、점막기층결손관도、낭상확장선체수，병검측혈청6-동전렬선소 F1α(6-K-PGF1α)、표피생장인자(EGF)、일양화담(NO)수평。결과여모형조비교，가미황기건중탕조대서궤양지수[(8.95±2.78)mm2비(20.82±5.12)mm2]、점막기층결손관도[(123.56±32.89)μm 비(229.32±49.69)μm]、낭상선체확장수[(1.65±0.76)개비(6.12±1.23)개]강저(P＜0.01)；재생점막후도[(329.55±32.22)μm 비(198.22±5.83)μm]、표면점액후도[(44.3±2.8)μm 비(24.5±7.8)μm]、혈청6-K-PGF1α[(93.7±8.9)pg/ml비(58.5±5.8)pg/ml]、EGF[(2.11±0.29)ng/L 비(0.82±0.20)ng/L]、NO[(0.51±0.03)μmol/L 비(0.22±0.05)μmol/L]수평승고(P＜0.01)；제궤양지수외，기타각항지표개선정황가미황기건중탕조균우우오미랍서조(P＜0.05)。결론가미황기건중탕가강저궤양지수、점막기층결손관도급낭상선체확장수，제고재생점막후도、점막표면점액후도급6-K-PGF1α、EGF、NO 수평，제고실험성위궤양유합질량。
Objective To investigate the effect of modified Huangqi-Jianzhong decoction on healing quality of gastric ulcer in rats and to explore its mechanism. Methods 40 Wistar rats were randomly divided into a normal group, a model group, a modified Huangqi-Jianzhong decoction group and a omeprazole group (n=10) according to the random number table method. These rats were used to model of chronic gastric ulcer induced by acetic acid 100%. On third day after surgery, the rats in the modified Huangqi-Jianzhong decoction group were treated with 10% of the modified Huangqi-Jianzhong decoction according to the dose of 20 ml per kilogram of body weight. The rats in the omeprazole group were treated with a 3% omeprazole suspension according to the of 20 ml per kilogram of body weight. The rats in the normal group and the model group were treated with the same volume of saline. After treated with corresponding treatment for 16 days, the gastric mucosa pathology, ulcer index, regenerative mucosal thickness, mucosal surface mucus thickness, muscularis mucosal layer width of the defect, cystically dilated glands number and level of serum prostaglandin (6-K-PGF1α), epidermal growth factor (EGF), nitric oxide (NO) were observed. Results Compared with the model group, the rat ulcer index (8.95 ± 2.78 mm2 vs. 20.82 ± 5.12 mm2), mucosal muscularis defect width (123.56 ± 32.89 μm vs. 229.32 ± 49.69 μm), cystic glandular expansion (1.65 ± 0.76 vs. 6.12 ± 1.23) were lower in the modified Huangqi-Jianzhong decoction group (P<0.01). Thickness of regenerated mucosa (329.55 ± 32.22 μm vs. 198.22 ± 5.83 μm), the surface thickness of mucus (44.3 ± 2.8 μm vs. 24.5 ± 7.8 μm), serum 6-K-PGF1α (93.7 ± 8.9 pg/ml vs. 58.5 ± 5.8 pg/ml), EGF (2.11 ± 0.29 ng/L vs. 0.82 ± 0.20 ng/L) , NO (0.51 ± 0.03 μmol/L vs. 0.22 ± 0.05 μmol/L) was higher (P<0.01). In addition to the ulcer index, other each target improvement flavored were better in the modified Huangqi-Jianzhong decoction group than in the omeprazole group (P<0.05). Conclusion Modified Huangqi-Jianzhong decoction can improve the quality of ulcer healing by promoting gastric mucosa ulcer repair, reducimg ulcer index, mucous muscularis layer defect width and cystic glandular expansion and improving the thickness of regenerative mucosa, mucosal surface mucus thickness and cyst, EGF, NO level and thus improve the experimental gastric ulcer healing quality.