CAJ | 학술논문

目的：初步研究二甲双胍（ metformin，Met）和Met联合顺铂（ cisplatin， Cisp）对人胃癌MKN-45细胞系的抗癌作用。方法应用不同浓度Met和Cisp单独或联合处理人胃癌MKN-45细胞系，MTT法分析细胞存活率，RT-PCR检测生存素、mTOR和Akt的mRNA表达水平，流式细胞仪检测细胞凋亡。结果单独使用Met可抑制增殖并诱导凋亡；Met联合Cisp时，可降低Cisp的抗增殖作用，且生存素、Akt和mTOR的mRNA表达明显增加（P＜0．05）。 Met对Cisp的拮抗作用可能是通过生存素、Akt和mTOR信号通路调节。结论 Met能抑制人胃癌MKN-45细胞系对Cisp化疗的敏感性，在患有2型糖尿病的胃癌患者中，应该慎重将Met与Cisp联合使用。
목적：초보연구이갑쌍고（ metformin，Met）화Met연합순박（ cisplatin， Cisp）대인위암MKN-45세포계적항암작용。방법응용불동농도Met화Cisp단독혹연합처리인위암MKN-45세포계，MTT법분석세포존활솔，RT-PCR검측생존소、mTOR화Akt적mRNA표체수평，류식세포의검측세포조망。결과단독사용Met가억제증식병유도조망；Met연합Cisp시，가강저Cisp적항증식작용，차생존소、Akt화mTOR적mRNA표체명현증가（P＜0．05）。 Met대Cisp적길항작용가능시통과생존소、Akt화mTOR신호통로조절。결론 Met능억제인위암MKN-45세포계대Cisp화료적민감성，재환유2형당뇨병적위암환자중，응해신중장Met여Cisp연합사용。
Objective To evaluate the effect of Metformin and Metformin combined with Cisplatin on human gastric MKN -45 cell line.Methods Treated the human gastric cancer MKN-45 cell line withof the Metformin alone or Metformin combined withCis-platinby different concentrations ,thenanalysed the cell survival rate by MTT ,checked mRNA levels of survivin , mTOR, and Akt by re-al-time PCR, and assessed the Cell apoptosis by flow cytometry .Results Metformin alone could inhibit theproliferation and induce apoptosis, but it diminish anti-proliferative effects of theCisplatin when they were used in combination .When MKN-45 cells were trea-ted with Metformin and Cisplatin , the expression of survivin and mTOR were increased .The antagonistic effect of the Metformin on the Cisplatinmight be through survivin , Akt and mTOR signaling pathways .Conclusions Met can inhibit the chemotherapy sensitivity of CispinMKN-45 human gastric cancer cell line .For gastric cancer patients with Type 2 Diabeteswho treated with Cisp , Met should be carefully used.