武警医学
武警醫學
무경의학
Medical Journal of the Chinese People's Armed Police Forces
2015年
10期
1010-1014
,共5页
林华刚%王博%张军%贾红蔚
林華剛%王博%張軍%賈紅蔚
림화강%왕박%장군%가홍위
糖尿病%胰岛素%骨密度%骨形态计量学%骨生物力学测试
糖尿病%胰島素%骨密度%骨形態計量學%骨生物力學測試
당뇨병%이도소%골밀도%골형태계량학%골생물역학측시
diabetic mellitus%insulin%bone mineral density%bone histomorphemetry%bone biomechanical test
目的：探讨胰岛素对于链脲佐菌素（ streptozotocin ，STZ）诱导的糖尿病大鼠骨量、骨转换状态和骨力学性能的影响。方法单次尾静脉注射STZ制造糖尿病大鼠模型，成模后分为糖尿病大鼠组（ DM）、胰岛素治疗组（ INS）与正常对照组（CON），8周后处死，测定3组大鼠血清骨钙素和尿Ⅰ型胶原氨基末端交联肽（NTx）／肌酐（Cr）比值，DEXA方法测定大鼠离体腰椎和股骨骨密度。骨形态计量学方法分析大鼠骨量和骨转换变化情况。采用股骨三点弯曲试验和腰椎压缩试验分析各组大鼠骨力学性能。结果与CON组相比，DM大鼠血清骨钙素和尿NTx／Cr比值降低，DM大鼠腰椎和股骨骨密度降低。与DM组大鼠相比，INS组大鼠血清骨钙素水平增高，尿NTx／Cr比值无变化，INS组大鼠腰椎和股骨骨密度显著增大（P＜0．01），但未达CON组水平。骨形态计量学分析提示与CON组相比，DM大鼠骨小梁骨量[TBV／TTV（12．06±2．48）％ vs （24．12±1．84）％， P＜0．01]减少，骨形成速率减低[（0．44±0．11） vs （0．78±0．14）μm／d， P＜0．01]。与DM组大鼠相比，INS组大鼠骨小梁骨量显著增加[TBV／TTV（19．75±2．43）％， P＜0．01]，骨形成速率亦增加[（0．70±0．16）μm／d， P＜0．01]。股骨三点弯曲试验显示，与CON组大鼠相比，DM组大鼠最大载荷、弹性载荷、弯曲能量、最大应力均显著减低（ P＜0．01）。与DM组相比，INS组上述各力学指标均显著增加（P＜0．01），但均未达CON组水平。椎体压缩实验提示与CON组大鼠相比，DM组大鼠最大载荷、弹性载荷、能量吸收、弹性模量均显著减低（ P＜0．01）。与DM组相比，INS组上述各指标亦显著增加，差异有统计学意义（ P＜0．01）。结论 DM大鼠表现为低转换型骨量减少，且骨力学性能下降，胰岛素治疗可以促进骨形成，增加骨量，且能改善DM大鼠的骨力学性能。
目的：探討胰島素對于鏈脲佐菌素（ streptozotocin ，STZ）誘導的糖尿病大鼠骨量、骨轉換狀態和骨力學性能的影響。方法單次尾靜脈註射STZ製造糖尿病大鼠模型，成模後分為糖尿病大鼠組（ DM）、胰島素治療組（ INS）與正常對照組（CON），8週後處死，測定3組大鼠血清骨鈣素和尿Ⅰ型膠原氨基末耑交聯肽（NTx）／肌酐（Cr）比值，DEXA方法測定大鼠離體腰椎和股骨骨密度。骨形態計量學方法分析大鼠骨量和骨轉換變化情況。採用股骨三點彎麯試驗和腰椎壓縮試驗分析各組大鼠骨力學性能。結果與CON組相比，DM大鼠血清骨鈣素和尿NTx／Cr比值降低，DM大鼠腰椎和股骨骨密度降低。與DM組大鼠相比，INS組大鼠血清骨鈣素水平增高，尿NTx／Cr比值無變化，INS組大鼠腰椎和股骨骨密度顯著增大（P＜0．01），但未達CON組水平。骨形態計量學分析提示與CON組相比，DM大鼠骨小樑骨量[TBV／TTV（12．06±2．48）％ vs （24．12±1．84）％， P＜0．01]減少，骨形成速率減低[（0．44±0．11） vs （0．78±0．14）μm／d， P＜0．01]。與DM組大鼠相比，INS組大鼠骨小樑骨量顯著增加[TBV／TTV（19．75±2．43）％， P＜0．01]，骨形成速率亦增加[（0．70±0．16）μm／d， P＜0．01]。股骨三點彎麯試驗顯示，與CON組大鼠相比，DM組大鼠最大載荷、彈性載荷、彎麯能量、最大應力均顯著減低（ P＜0．01）。與DM組相比，INS組上述各力學指標均顯著增加（P＜0．01），但均未達CON組水平。椎體壓縮實驗提示與CON組大鼠相比，DM組大鼠最大載荷、彈性載荷、能量吸收、彈性模量均顯著減低（ P＜0．01）。與DM組相比，INS組上述各指標亦顯著增加，差異有統計學意義（ P＜0．01）。結論 DM大鼠錶現為低轉換型骨量減少，且骨力學性能下降，胰島素治療可以促進骨形成，增加骨量，且能改善DM大鼠的骨力學性能。
목적：탐토이도소대우련뇨좌균소（ streptozotocin ，STZ）유도적당뇨병대서골량、골전환상태화골역학성능적영향。방법단차미정맥주사STZ제조당뇨병대서모형，성모후분위당뇨병대서조（ DM）、이도소치료조（ INS）여정상대조조（CON），8주후처사，측정3조대서혈청골개소화뇨Ⅰ형효원안기말단교련태（NTx）／기항（Cr）비치，DEXA방법측정대서리체요추화고골골밀도。골형태계량학방법분석대서골량화골전환변화정황。채용고골삼점만곡시험화요추압축시험분석각조대서골역학성능。결과여CON조상비，DM대서혈청골개소화뇨NTx／Cr비치강저，DM대서요추화고골골밀도강저。여DM조대서상비，INS조대서혈청골개소수평증고，뇨NTx／Cr비치무변화，INS조대서요추화고골골밀도현저증대（P＜0．01），단미체CON조수평。골형태계량학분석제시여CON조상비，DM대서골소량골량[TBV／TTV（12．06±2．48）％ vs （24．12±1．84）％， P＜0．01]감소，골형성속솔감저[（0．44±0．11） vs （0．78±0．14）μm／d， P＜0．01]。여DM조대서상비，INS조대서골소량골량현저증가[TBV／TTV（19．75±2．43）％， P＜0．01]，골형성속솔역증가[（0．70±0．16）μm／d， P＜0．01]。고골삼점만곡시험현시，여CON조대서상비，DM조대서최대재하、탄성재하、만곡능량、최대응력균현저감저（ P＜0．01）。여DM조상비，INS조상술각역학지표균현저증가（P＜0．01），단균미체CON조수평。추체압축실험제시여CON조대서상비，DM조대서최대재하、탄성재하、능량흡수、탄성모량균현저감저（ P＜0．01）。여DM조상비，INS조상술각지표역현저증가，차이유통계학의의（ P＜0．01）。결론 DM대서표현위저전환형골량감소，차골역학성능하강，이도소치료가이촉진골형성，증가골량，차능개선DM대서적골역학성능。
Objective To study the effect of insulin on bone mass and the condition of bone turnover and bone biomechanical properties in STZ-induced diabetic rats .Methods Of 45 male SD rats studied , 30 were made diabetic by intravenous injection of streptozocin(50 mg/kg) on day-7.Thirty diabetic rats were randomly divided into 2 groups as group DM and control group .DM group were injected subcutaneously with insulin (6 U/kg/d) regularly.After their sacrifice on the day 56, blood was sampled and serum was separated for the measurement of Ca , P, ALPand OC.The left tibia was dissected for bone histomorphometry analysis .Right femur and lumbar vertebrae(L1-L4) were reserved for BMD test.The left femor was separated for three-point bending test,while the fifth lumbar vertibra was prepared for compression test to reflect the biomechanical properties of bone .Results A low-turnover osteopenia was evi-denced in diabetic rats by decreased BMD in both femur and lumbar vertebrae , reduced serum OC level , decreased urine NTx/Cr rati-o, decreased trabecular volume [TBV/TTV(12.06 ±2.48) vs (24.12 ±1.84)%, P<0.01]and bone formation rate[(0.44 ±0.11) vs (0.78 ±0.14)μm/d, P<0.01] by bone dynamic study .All these abnormalities except urine NTx/Cr ratio were partly or com-pletely normalized by insulin treatment.The bone densities in vertebrae and femur of diabetic rats significantly decreased (P<0.01). Maximal load, elastic load, bending energy , maximal stress in three-point bending test were conducted ,compared with normal control rats.These abnormalities could be partially corrected by insulin teatment (P<0.01).In addition,the fifth lumbar vertibrae of diabetic rats were obviously decreased(P<0.01), and maximal load,elastic load, energy absorbability and elastic modulus during compressing test.By insulin treatment, these indices partially reversed to the nor-mal control level(P<0.01).Conclusion A low-turnover osteope-nia is evidenced in STZ-induced diabetic rats with impaired bone bio-mechanical abilities .All these abnormalities of bone can be partially corrected by the injection of insulin .It shows that insulin can stim-ulate bone formation dramatically while not influence osteoclastic activity in diabetic rats .
