CAJ | 학술논문

植物的生物钟节律可以被环境中的光／黑暗以及冷／热循环所诱导，并使其与环境同步。植物生物钟由输入途径、中央振荡器、输出途径组成。目前对植物生物钟的研究已经揭示生物钟最基本的组成，但是关于生物钟的运作机理及网络还需要进一步研究。CONSTANS LIKE 7（COL7）是 CONSTANS（CO）的家族基因。以拟南芥野生型（wild type，WT）、突变体 col7以及 COL7过量表达转基因株系 COL7-OX-10和 COL7-OX-11为材料，利用定量 PCR、叶片运动等方法，分析 COL7是否受生物钟调控以及 COL7是否参与调控生物。实验结果显示：COL7不仅受生物钟调控，同时也参与调控生物钟。
식물적생물종절률가이피배경중적광／흑암이급랭／열순배소유도，병사기여배경동보。식물생물종유수입도경、중앙진탕기、수출도경조성。목전대식물생물종적연구이경게시생물종최기본적조성，단시관우생물종적운작궤리급망락환수요진일보연구。CONSTANS LIKE 7（COL7）시 CONSTANS（CO）적가족기인。이의남개야생형（wild type，WT）、돌변체 col7이급 COL7과량표체전기인주계 COL7-OX-10화 COL7-OX-11위재료，이용정량 PCR、협편운동등방법，분석 COL7시부수생물종조공이급 COL7시부삼여조공생물。실험결과현시：COL7불부수생물종조공，동시야삼여조공생물종。
The circadian clock of plants can be trained by light/dark and cold /hot circles,and make it synchronized-with the environment.The circadian clock of plants is composed of input pathway,oscillator central and output pathway. The present study of circadian clock has revealed the basic composition of circadian clock,butthe operation mechanism and network of circadian clock need further research.CONSTANS LIKE 7(COL7)is one of the CONSTANS(CO)family genes.We used the real-time PCR,leaf movement to analyze the circadian clock of wild type (WT),col7 mutant and COL7 overexpression lines (COL7-OX-1 0 and COL7-OX-1 1 ).The results showed that COL7 is regulated by circadian clock and also involved in regulating circadian clock.