CAJ | 학술논문

目的：本文主要以金鱼中的 dvl-3基因为研究对象，研究 dvl-3在金鱼不同的组织和胚胎发育时期中可能行使的功能。方法：采用 RACE 克隆技术获得金鱼 dvl-3基因的 cDNA 全长，再利用半定量 RT-PCR 技术和蛋白免疫印记（Western blot）技术分析研究 dvl-3基因在 mRNA 水平和蛋白水平上的表达差异。结果：在金鱼不同组织与胚胎发育时期，dvl-3基因在 mRNA 水平和蛋白水平上具有显著的表达差异性。结论：由此可见散乱蛋白dvl-3在金鱼不同的组织和胚胎发育时期中可能行使不同的功能，但其具体的功能及机制还有待进一步研究。
목적：본문주요이금어중적 dvl-3기인위연구대상，연구 dvl-3재금어불동적조직화배태발육시기중가능행사적공능。방법：채용 RACE 극륭기술획득금어 dvl-3기인적 cDNA 전장，재이용반정량 RT-PCR 기술화단백면역인기（Western blot）기술분석연구 dvl-3기인재 mRNA 수평화단백수평상적표체차이。결과：재금어불동조직여배태발육시기，dvl-3기인재 mRNA 수평화단백수평상구유현저적표체차이성。결론：유차가견산란단백dvl-3재금어불동적조직화배태발육시기중가능행사불동적공능，단기구체적공능급궤제환유대진일보연구。
Objective:Here,our study focused on gene dvl-3 in Carassius auratus.Research the function of dvl-3 pro-teins during embryonic development stages in Carassius auratus as well as in different tissues.Method:Firstly,we used RACE (rapid-amplification of cDNA ends)clone technology to obtain full-length cDNA of gene dvl-3,and then semi-quantitative RT-PCR and western blot were used to analyze the differential expression of dvl-3 in mRNA level and protein level respectively.Results:The results showed that:Semi-quantitative RT-PCR and semi-quantitative western blot re-sults are different in different embryonic development and different tissues.Conclusions:Thus it showed that the func-tion of dvl-3 proteins varied during embryonic development stages in Carassius auratus as well as in different tissues. However,the exact mechanism remained to be further explored.