CAJ | 학술논문

目的：建立泽兰水提物中主要成分咖啡酸、迷迭香酸、熊果酸、芦丁、木犀草素、齐墩果酸和槲皮素快速测定的超高效液相色谱‐电喷雾‐三重四级杆串联质谱方法。方法超声法制备泽兰水提物，XBridge‐C18（100 mm ×2．1 mm ，3．5μm）反相梯度洗脱法对样品进行分离；电喷雾电离源负离子模式多级反应监测扫描法对各成分进行定量分析。结果方法的相对标准偏差在0．24％～2．8％范围内，回收率在93．3％～105．2％之间，检测限为0．05～1．0μg · L －1。结论该方法具有分析速度快，灵敏度高和特异性强的特点，可为中药中目标成分的含量测定提供方法学参考。
목적：건립택란수제물중주요성분가배산、미질향산、웅과산、호정、목서초소、제돈과산화곡피소쾌속측정적초고효액상색보‐전분무‐삼중사급간천련질보방법。방법초성법제비택란수제물，XBridge‐C18（100 mm ×2．1 mm ，3．5μm）반상제도세탈법대양품진행분리；전분무전리원부리자모식다급반응감측소묘법대각성분진행정량분석。결과방법적상대표준편차재0．24％～2．8％범위내，회수솔재93．3％～105．2％지간，검측한위0．05～1．0μg · L －1。결론해방법구유분석속도쾌，령민도고화특이성강적특점，가위중약중목표성분적함량측정제공방법학삼고。
Objective To develop a method for rapid and simultaneous determination of rosmarinci acid ,caffeic acid ,ursolic acid ,cya‐nidenon ,phytomelin ,oleanolic acid and quercetin by ultra‐performance liquid chromatography‐electrospray‐triple quadrupole mass spectrometer .Methods Ultrasonic method was used to prepare the aqueous extract of Lycopus lucidus Turcz .;an XBridge‐C18re‐verse phase column was employed to separate the interest compounds through gradient elution ;multi reaction monitoring method under electrospray ionization and negative ion mode was utilized to determine the seven compounds .Results The relative standard deviations of the method were in the range of 0 .24%‐2 .8% ;the recoveries were ranged from 93 .3%‐105 .2% ;the limits of detec‐tion were 0 .05‐1 .0 μg · L -1 .Conclusion The proposed method has the advantages of rapid speed of analysis ,high sensitivity and specificity .It is possible to become an alternative for the determination of target compounds in traditional Chinese medicine .