CAJ | 학술논문

目的：观察活血及活血解毒配伍对急性心肌梗死大鼠心肌损伤的保护作用。方法选择雄性SD大鼠100只，随机分为：假手术组（等量生理盐水）、模型组（等量生理盐水）、活血组[芎芍胶囊0．39 g／（kg · d ）]、活血解毒组[芎芍胶囊0．39 g／（kg · d）＋黄连胶囊0．135 g／（kg · d）]、美托洛尔组[美托洛尔2．25 mg／（kg · d）]，每组20只。造模24 h处死大鼠，观察心肌组织病理改变，T TC法测量心肌梗死面积，ELISA 法检测血清肌酸激酶同工酶（CK‐MB）、肌钙蛋白I（cTnI）、内皮素1、血管性假血友病因子（vWF）、血栓调节蛋白（TM ）等指标。结果与假手术组比较，模型组心肌梗死面积、CK‐MB、cTnI、内皮素1、vWF、TM 明显升高（P＜0．01）。与美托洛尔组比较，活血解毒组cTnI明显降低[（11．88±1．98）pg／ml vs （14．13±2．02）pg／ml]，内皮素1、vWF明显降低（P＜0．05，P＜0．01）。与活血组比较，活血解毒组cTnI、内皮素1、vWF、TM 明显改善（P＜0．05，P＜0．01）。结论活血解毒配伍对心肌损伤发挥了较好的保护作用，显著优于单纯的活血化瘀。
목적：관찰활혈급활혈해독배오대급성심기경사대서심기손상적보호작용。방법선택웅성SD대서100지，수궤분위：가수술조（등량생리염수）、모형조（등량생리염수）、활혈조[궁작효낭0．39 g／（kg · d ）]、활혈해독조[궁작효낭0．39 g／（kg · d）＋황련효낭0．135 g／（kg · d）]、미탁락이조[미탁락이2．25 mg／（kg · d）]，매조20지。조모24 h처사대서，관찰심기조직병리개변，T TC법측량심기경사면적，ELISA 법검측혈청기산격매동공매（CK‐MB）、기개단백I（cTnI）、내피소1、혈관성가혈우병인자（vWF）、혈전조절단백（TM ）등지표。결과여가수술조비교，모형조심기경사면적、CK‐MB、cTnI、내피소1、vWF、TM 명현승고（P＜0．01）。여미탁락이조비교，활혈해독조cTnI명현강저[（11．88±1．98）pg／ml vs （14．13±2．02）pg／ml]，내피소1、vWF명현강저（P＜0．05，P＜0．01）。여활혈조비교，활혈해독조cTnI、내피소1、vWF、TM 명현개선（P＜0．05，P＜0．01）。결론활혈해독배오대심기손상발휘료교호적보호작용，현저우우단순적활혈화어。
Objective To study the role of promoting blood circulation and its combination with de‐toxication in protecting AMI rats against myocardial injury .Methods One hundred male SD rats were randomly divided into sham operation group ,model group ,promoting blood circulation group ,promoting blood circulation and detoxication group ,and metoprolol treatment group (20 in each group) .The animals were sacrificed 24 h after a model of AMI was established .Their myo‐cardial tissue lesions were observed by optical microscopy ,their myocardial infarction size was measured by TIC ,their serum levels of CK‐MB ,cTnI ,ET‐1 ,vWF ,and TM were measured by ELISA .Results The myocardial infarction size was significantly larger and the serum levels of CK‐MB ,cTnI ,ET‐1 ,vWF ,and TM were significantly higher in model group than in sham opera‐tion group (P<0 .01) .The serum levels of cTnI ,ET‐1 and vWF were significantly lower in pro‐moting blood circulation and detoxication group than in metoprolol treatment group ( P<0 .05 , P<0 .01) .The serum levels of cTnI ,ET‐1 ,vWF ,and TM were improved more signficantly in promoting blood circulation and detoxication group than in promoting blood circulation group (P<0 .05 ,P< 0 .01) .Conclusion Combined promoting blood circulation and detoxication can protect AMI rats against myocardial injury ,and its effect is significantly better than that of simple promoting blood circulation to remove blood stasis .