武警医学
武警醫學
무경의학
Medical Journal of the Chinese People's Armed Police Forces
2015年
9期
895-899
,共5页
朱彪%赵刚%徐红梅%郭希民%郭红延
硃彪%趙剛%徐紅梅%郭希民%郭紅延
주표%조강%서홍매%곽희민%곽홍연
骨髓间充质干细胞%组织工程骨%口腔种植
骨髓間充質榦細胞%組織工程骨%口腔種植
골수간충질간세포%조직공정골%구강충식
bone marrow mesenchymal stem cells%tissue-engineered bones%oral implantology
目的：评价以骨髓间充质干细胞（bone marrow mesenchymal stem cells ，BMSCs）为基础构建组织工程骨修复种植体周围骨缺损的成骨效果。方法在3只犬双侧下颌前磨牙区各植入2颗种植体，分为对照组和实验组，每组6颗种值体；在种植体远中制备骨缺损，对照组每侧骨缺损区随机植入富血小板血浆（ platelet－rich plasma，PRP）和实验组成骨诱导培养BM－SCs复合PRP。分别于移植后1、3个月取材，行大体、影像学及组织学观察。结果1个月后，实验组可见较多的骨基质，较少的胶原纤维。实验组骨密度值大于对照组（101±8．99）vs（93±9．81），差异有统计学意义（P＜0．05）。3个月后，实验组可见较多的哈弗系统，实验组与对照组骨密度值变化不大（100．75±12．05）vs（105±11．58），差异无统计学意义。结论以BMSCs为基础的组织工程骨能在种植体周围较好地成骨。
目的：評價以骨髓間充質榦細胞（bone marrow mesenchymal stem cells ，BMSCs）為基礎構建組織工程骨脩複種植體週圍骨缺損的成骨效果。方法在3隻犬雙側下頜前磨牙區各植入2顆種植體，分為對照組和實驗組，每組6顆種值體；在種植體遠中製備骨缺損，對照組每側骨缺損區隨機植入富血小闆血漿（ platelet－rich plasma，PRP）和實驗組成骨誘導培養BM－SCs複閤PRP。分彆于移植後1、3箇月取材，行大體、影像學及組織學觀察。結果1箇月後，實驗組可見較多的骨基質，較少的膠原纖維。實驗組骨密度值大于對照組（101±8．99）vs（93±9．81），差異有統計學意義（P＜0．05）。3箇月後，實驗組可見較多的哈弗繫統，實驗組與對照組骨密度值變化不大（100．75±12．05）vs（105±11．58），差異無統計學意義。結論以BMSCs為基礎的組織工程骨能在種植體週圍較好地成骨。
목적：평개이골수간충질간세포（bone marrow mesenchymal stem cells ，BMSCs）위기출구건조직공정골수복충식체주위골결손적성골효과。방법재3지견쌍측하합전마아구각식입2과충식체，분위대조조화실험조，매조6과충치체；재충식체원중제비골결손，대조조매측골결손구수궤식입부혈소판혈장（ platelet－rich plasma，PRP）화실험조성골유도배양BM－SCs복합PRP。분별우이식후1、3개월취재，행대체、영상학급조직학관찰。결과1개월후，실험조가견교다적골기질，교소적효원섬유。실험조골밀도치대우대조조（101±8．99）vs（93±9．81），차이유통계학의의（P＜0．05）。3개월후，실험조가견교다적합불계통，실험조여대조조골밀도치변화불대（100．75±12．05）vs（105±11．58），차이무통계학의의。결론이BMSCs위기출적조직공정골능재충식체주위교호지성골。
Objective To repair bone defect in implant region using bone marrow mesenchymal stem cells -based tissue-engi-neering bone and investigate its osteogenesis potential .Methods Bilateral implantaion ,two implants every side , was performed in the mandible premolar region of 3 Beagles and bone defects were created in the distal of every implant .Two bone defects in one side of mandible premolar region were randomly repaired with two types of grafts :PRP (n=6, the control group);and compound osteogenic-induced BMSCs with PRP(n=6, the experimental group).1 and 3 months after transplation, gross investigation,X-ray examination and histological observation were conducted to examine bone formation .Results One month after implantation ,the osteogenesis in ex-perimental group was better than in the control group .We detected more bone matrix and less collagenous fibers in the experimental group.The bone density in the experimental group was higher than in the control group and the difference was significantly different [(101 ±8.99)vs(93 ±9.81),P≤0.05].Three months after implantation,the bone density in the experimental group tended to be higher than in the control group;however,the difference was not statistically significant [(100.75 ±12.05)vs(105 ±11.58)].But we detected more Haversian system in the experimental group .Conclusion Bone marrow stem cell-based tissue-engineered bone has high osteogenic potential around implants .
