CAJ | 학술논문

目的探讨低氧环境下红景天与角质细胞生长因子/缺氧诱导因子( KGF/HIF)修饰的骨髓间充质干细胞( MSCs)联合应用对大鼠脊髓损伤( SCI)修复的影响. 方法低氧条件下制备大鼠SCI模型,模型制备成功随机分为4组,对照组给予生理盐水,红景天组给予红景天胶囊,移植组移植KGF/HIF修饰的MSCs,联合应用组同时给予红景天及干细胞移植,评价4组大鼠运动能力恢复情况,观察损伤组织病理学及脊髓神经细胞变化. 结果移植组、红景天组和联合应用组大鼠联合行为学评分均高于对照组( P<0. 05 );脊髓损伤组织细胞数目增多,神经细胞坏死减少,HIF表达水平升高,尤以联合应用组变化明显. 结论红景天联合KGF/HIF修饰的骨髓MSCs可促进SCI修复.
목적 탐토저양배경하홍경천여각질세포생장인자/결양유도인자( KGF/HIF)수식적골수간충질간세포( MSCs)연합응용대대서척수손상( SCI)수복적영향. 방법 저양조건하제비대서SCI모형,모형제비성공수궤분위4조,대조조급여생리염수,홍경천조급여홍경천효낭,이식조이식KGF/HIF수식적MSCs,연합응용조동시급여홍경천급간세포이식,평개4조대서운동능력회복정황,관찰손상조직병이학급척수신경세포변화. 결과 이식조、홍경천조화연합응용조대서연합행위학평분균고우대조조( P<0. 05 );척수손상조직세포수목증다,신경세포배사감소,HIF표체수평승고,우이연합응용조변화명현. 결론 홍경천연합KGF/HIF수식적골수MSCs가촉진SCI수복.
Objective To investigate the effect of Rhodiola Rosea combined with kinetics gain factor (KGF) and hypoxia-inducible factor 1 (HIF-1) mediated bone marrow mesenchymal stem cells (MSCs) under hypoxia condition in repair of spinal cord injuries ( SCI) in rats. Methods The SCI rat models were established under hypoxia condition and were randomly divided into 4 groups after successful establishment. The control group was treated with physiological saline;the Rhodiola Rosea group was treated with Rhodiola Rosea capsules; the transplantation group was transplanted with rats' bone marrow MSCs modulated by KGF and HIF;the combination group was transplanted with rats' bone marrow MSCs modulated by KGF, HIF and Rhodiola Rosea. The recovery of motor ability of models was evaluated in each group, and the changes of histopathology and spinal nerve cells were observed in each group. Results In transplantation, Rhod-iola Rosea and combination groups, the combined ethology scores were higher (P<0. 05), SCI histiocytes numbers were larger, numbers of neural apoptosis were decreased, and HIF expression levels were increased higher than those of control group;the changes were more obvious in combination group. Conclusion Rhodiola rosea combined with KGF and HIF may promote the SCI repair modulated by bone marrow MSCs.