中华老年心脑血管病杂志
中華老年心腦血管病雜誌
중화노년심뇌혈관병잡지
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2015年
9期
968-971
,共4页
张运周%陈卫碧%高岱全%刘刚%宿英英
張運週%陳衛碧%高岱全%劉剛%宿英英
장운주%진위벽%고대전%류강%숙영영
脑缺血%组蛋白赖氨酸N-甲基转移酶%脑缺血发作 ,短暂性%免疫组织化学%海马%再灌注损伤
腦缺血%組蛋白賴氨痠N-甲基轉移酶%腦缺血髮作 ,短暫性%免疫組織化學%海馬%再灌註損傷
뇌결혈%조단백뢰안산N-갑기전이매%뇌결혈발작 ,단잠성%면역조직화학%해마%재관주손상
brain ischemia%histone-lysine N-methyltransferase%ischemic attack,transient%immuno-histochemistry%hippocampus%reperfusion injury
目的:探讨大鼠全脑缺血损伤前后脑内是否存在特异性组蛋白赖氨酸去甲基酶1(LSD1)及其时间、空间分布规律。方法选择成年SD大鼠126只,免疫组织化学和Western blot各63只制作短暂性全脑缺血模型,大鼠按脑缺血再灌注后不同处死时间随机分为实验组56只(1、6、12 h ,1、3、7、14、30 d),每个时间点7只,对照组7只。用免疫组织化学及Western blot ,观察大鼠LSD1在各脑区的表达。结果免疫组织化学及Western blot结果一致显示,LSD1在缺血前散在分布各脑区,主要分布于海马及前额区皮质。实验组缺血再灌注后1 h LSD1免疫反应阳性细胞明显增高,于6 h达到小高峰,随后缓慢下降,至缺血再灌注后12 h达低点,但齿状回区及CA1区仍明显高于对照组(P<0.05)。缺血再灌注12 h后,不同脑区表现出各自的时间分布特点:齿状回区1 d、海马CA1区3 d及前额区7 d LSD1第2高峰分别为[(13492.7±639.6)个 vs (614.1±126.6)个,(2371.1±403.2)个 vs (119.3±22.6)个,(1874.7±78.1)个 v s (97.4±15.8)个,P<0.01],随后缓慢下降,直至30 d恢复到对照组水平。结论正常状态下,LSD1即存在于大鼠海马齿状回区、CA1区及前额区,并在缺血性脑损伤后呈现不同的时间变化特点。从空间分布、时间变化特点推测,LSD1可能在脑损伤后诸多调节通路中起重要的调节作用。
目的:探討大鼠全腦缺血損傷前後腦內是否存在特異性組蛋白賴氨痠去甲基酶1(LSD1)及其時間、空間分佈規律。方法選擇成年SD大鼠126隻,免疫組織化學和Western blot各63隻製作短暫性全腦缺血模型,大鼠按腦缺血再灌註後不同處死時間隨機分為實驗組56隻(1、6、12 h ,1、3、7、14、30 d),每箇時間點7隻,對照組7隻。用免疫組織化學及Western blot ,觀察大鼠LSD1在各腦區的錶達。結果免疫組織化學及Western blot結果一緻顯示,LSD1在缺血前散在分佈各腦區,主要分佈于海馬及前額區皮質。實驗組缺血再灌註後1 h LSD1免疫反應暘性細胞明顯增高,于6 h達到小高峰,隨後緩慢下降,至缺血再灌註後12 h達低點,但齒狀迴區及CA1區仍明顯高于對照組(P<0.05)。缺血再灌註12 h後,不同腦區錶現齣各自的時間分佈特點:齒狀迴區1 d、海馬CA1區3 d及前額區7 d LSD1第2高峰分彆為[(13492.7±639.6)箇 vs (614.1±126.6)箇,(2371.1±403.2)箇 vs (119.3±22.6)箇,(1874.7±78.1)箇 v s (97.4±15.8)箇,P<0.01],隨後緩慢下降,直至30 d恢複到對照組水平。結論正常狀態下,LSD1即存在于大鼠海馬齒狀迴區、CA1區及前額區,併在缺血性腦損傷後呈現不同的時間變化特點。從空間分佈、時間變化特點推測,LSD1可能在腦損傷後諸多調節通路中起重要的調節作用。
목적:탐토대서전뇌결혈손상전후뇌내시부존재특이성조단백뢰안산거갑기매1(LSD1)급기시간、공간분포규률。방법선택성년SD대서126지,면역조직화학화Western blot각63지제작단잠성전뇌결혈모형,대서안뇌결혈재관주후불동처사시간수궤분위실험조56지(1、6、12 h ,1、3、7、14、30 d),매개시간점7지,대조조7지。용면역조직화학급Western blot ,관찰대서LSD1재각뇌구적표체。결과면역조직화학급Western blot결과일치현시,LSD1재결혈전산재분포각뇌구,주요분포우해마급전액구피질。실험조결혈재관주후1 h LSD1면역반응양성세포명현증고,우6 h체도소고봉,수후완만하강,지결혈재관주후12 h체저점,단치상회구급CA1구잉명현고우대조조(P<0.05)。결혈재관주12 h후,불동뇌구표현출각자적시간분포특점:치상회구1 d、해마CA1구3 d급전액구7 d LSD1제2고봉분별위[(13492.7±639.6)개 vs (614.1±126.6)개,(2371.1±403.2)개 vs (119.3±22.6)개,(1874.7±78.1)개 v s (97.4±15.8)개,P<0.01],수후완만하강,직지30 d회복도대조조수평。결론정상상태하,LSD1즉존재우대서해마치상회구、CA1구급전액구,병재결혈성뇌손상후정현불동적시간변화특점。종공간분포、시간변화특점추측,LSD1가능재뇌손상후제다조절통로중기중요적조절작용。
Objective To study the temporal and spatial distribution of lysine‐specific demethylase 1 (LSD1)in rats following transient global ischemia .Methods One hundred and twenty‐six adult SD rats were included in this study .Sixty‐three of the animals were randomly divided into 8 (1 ,6 , 12 h and 1 ,3 ,7 ,14 ,30 d) experimental groups (7 in each group) and control group (n=7) .Ex‐pression of LSD1 in different sites of rat brain was detected by immunohistochemistry and West‐ern blot ,respectively ,after a model of transient global ischemia was established .Results Immu‐nohistochemistry and Western blot showed that LSD1 was mainly distributed in hippocampus and frontal cortex of rats .The number of positive immunoreactive cells to LSD1 in experimental groups increased significantly at 1 h and reached its peak at 6 h after I/R ,then decreased slowly and reached its lowest point at 12 h after I/R ,but was significantly greater in dentate gyrus and CA1 of enperimental groups than in those of control group (P<0 .05) .However ,the number of positive immunoreactive cells to LSD1 in dentate gyrus ,CAI and frontal cortex was significantly greater in experimental groups than in control group at 12 h after I/R (P<0 .01) ,reached its sec‐ond peak on days 1 ,3 ,7 ,then decreased slowly and returned to that in control group on day 30 af‐ter after I/R .Conclusion LSD1 in dentate gyrus ,CA1 ,and frontal cortex of rats plays an impor‐tant role in regulating the pathways following cerebral ischemia .