中华老年心脑血管病杂志
中華老年心腦血管病雜誌
중화노년심뇌혈관병잡지
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2015年
9期
964-967
,共4页
乙酰半胱氨酸%造影剂%C反应蛋白质%肿瘤坏死因子α%肾功能衰竭 ,急性%丙二醛%超氧化物歧化酶
乙酰半胱氨痠%造影劑%C反應蛋白質%腫瘤壞死因子α%腎功能衰竭 ,急性%丙二醛%超氧化物歧化酶
을선반광안산%조영제%C반응단백질%종류배사인자α%신공능쇠갈 ,급성%병이철%초양화물기화매
acetylcysteine%contrast media%C-reactive protein%tumor necrosis factor-alpha%kidney failure,acute%malondialdehyde%superoxide dismutase
目的：探讨N‐乙酰半胱氨酸（N A C ）对大鼠造影剂诱导的急性肾损伤的保护作用及其机制。方法选择成年Wistar大鼠45只，随机分为对照组、模型组和NAC组，每组15只。注射造影剂前和注射造影剂后48、72 h分别采血2 ml检测血清肌酐、C反应蛋白、TNF‐α水平。注射造影剂后72 h处死全部大鼠，测量肾脏组织中丙二醛、超氧化物歧化酶（SOD）和NO的水平，检测Bax和Bcl‐2蛋白表达。结果模型组和NAC组注射造影剂后48 h肌酐[（36．98±3．70）μmol／L ，（33．04±2．95）μmol／L vs （28．36±3．06）μmol／L]、72 h C反应蛋白[（1．56±0．18）μg／L ，（1．38±0．19）μg／L v s （1．11±0．19）μg／L ]显著高于对照组，T N F‐α、丙二醛和Bax蛋白表达水平明显高于对照组，且模型组明显高于NAC组（P＜0．05）；模型组和NAC组注射造影剂后SOD、NO、Bcl‐2蛋白表达明显低于对照组，且模型组明显低于NAC组（P＜0．05）；模型组和NAC组大鼠注射造影剂后免疫组织化学中观察转化生长因子β1表达较对照组增多，但NAC组转化生长因子β1表达低于模型组。结论 NAC对大鼠肾脏有一定的保护作用，可减少造影剂诱导的急性肾损伤发生，可能与其抗炎症、抗氧化、抗凋亡、抗细胞因子等作用有关。
目的：探討N‐乙酰半胱氨痠（N A C ）對大鼠造影劑誘導的急性腎損傷的保護作用及其機製。方法選擇成年Wistar大鼠45隻，隨機分為對照組、模型組和NAC組，每組15隻。註射造影劑前和註射造影劑後48、72 h分彆採血2 ml檢測血清肌酐、C反應蛋白、TNF‐α水平。註射造影劑後72 h處死全部大鼠，測量腎髒組織中丙二醛、超氧化物歧化酶（SOD）和NO的水平，檢測Bax和Bcl‐2蛋白錶達。結果模型組和NAC組註射造影劑後48 h肌酐[（36．98±3．70）μmol／L ，（33．04±2．95）μmol／L vs （28．36±3．06）μmol／L]、72 h C反應蛋白[（1．56±0．18）μg／L ，（1．38±0．19）μg／L v s （1．11±0．19）μg／L ]顯著高于對照組，T N F‐α、丙二醛和Bax蛋白錶達水平明顯高于對照組，且模型組明顯高于NAC組（P＜0．05）；模型組和NAC組註射造影劑後SOD、NO、Bcl‐2蛋白錶達明顯低于對照組，且模型組明顯低于NAC組（P＜0．05）；模型組和NAC組大鼠註射造影劑後免疫組織化學中觀察轉化生長因子β1錶達較對照組增多，但NAC組轉化生長因子β1錶達低于模型組。結論 NAC對大鼠腎髒有一定的保護作用，可減少造影劑誘導的急性腎損傷髮生，可能與其抗炎癥、抗氧化、抗凋亡、抗細胞因子等作用有關。
목적：탐토N‐을선반광안산（N A C ）대대서조영제유도적급성신손상적보호작용급기궤제。방법선택성년Wistar대서45지，수궤분위대조조、모형조화NAC조，매조15지。주사조영제전화주사조영제후48、72 h분별채혈2 ml검측혈청기항、C반응단백、TNF‐α수평。주사조영제후72 h처사전부대서，측량신장조직중병이철、초양화물기화매（SOD）화NO적수평，검측Bax화Bcl‐2단백표체。결과모형조화NAC조주사조영제후48 h기항[（36．98±3．70）μmol／L ，（33．04±2．95）μmol／L vs （28．36±3．06）μmol／L]、72 h C반응단백[（1．56±0．18）μg／L ，（1．38±0．19）μg／L v s （1．11±0．19）μg／L ]현저고우대조조，T N F‐α、병이철화Bax단백표체수평명현고우대조조，차모형조명현고우NAC조（P＜0．05）；모형조화NAC조주사조영제후SOD、NO、Bcl‐2단백표체명현저우대조조，차모형조명현저우NAC조（P＜0．05）；모형조화NAC조대서주사조영제후면역조직화학중관찰전화생장인자β1표체교대조조증다，단NAC조전화생장인자β1표체저우모형조。결론 NAC대대서신장유일정적보호작용，가감소조영제유도적급성신손상발생，가능여기항염증、항양화、항조망、항세포인자등작용유관。
Objective To study the role of N‐acetylcysteine (NAC) in protecting rats against con‐trast‐induced acute kidney injury .Methods Forty‐five adult Wistar rats were randomly divided into control group ,model group and NAC group (15 in each group) .Their serum levels of creati‐nine ,CRP ,TNF‐a were measured before ,and 48 and 72 h after contrast medium injection .The an‐imals were killed 72 h after contrast medum injection .Their serum levels of malondialdehyde , SOD ,NO were measured .Eexpressions of Bax and Bcl‐2 were detected .Results The serum levels of creatinine ,CRP ,TNF‐a ,malondialdehyde and the expression level of Bax were significantly higher in model group and NAC group than in control group ,and in model group than in NAC group after contrast injection (P< 0 .05) .The serum levels of SOD and NO and the expression level of Bcl‐2 were significantly lower in model group and NAC group than in control group ,and in model group than in NAC group after constrast injection (P< 0 .05) .Immunohistochemistry demonstrated that the TGFβ1 expression level was significantly higher in model group and NAC group than in control group and was significantly lower in NAC group than in model group .Con‐clusion NAC can protect rats against kidney injury and reduce contrast‐induced acute kidney in‐jury by inhibiting inflammation ,oxidation ,apoptosis and cytokines .
