CAJ | 학술논문

目的：探讨中国上海松江地区汉族人群烟酰胺腺嘌呤二核苷酸磷酸[NAD （P）H ]氧化酶 p22phox 亚基C242T基因多态性与颈动脉硬化的相关性。方法选择上海松江地区颈动脉硬化患者129例作为颈动脉硬化组，同期健康体检者143例作为对照组，采用病例对照研究，联合聚合酶链反应‐限制性片段长度多态性分析、基因测序技术，对2组C242T基因多态性位点进行基因型、等位基因频率进行分析。结果颈动脉硬化组C242T基因CT＋TT基因型频率及T等位基因频率显著高于对照组（10．1％ vs 2．8％，P＝0．028；5．4％ vs 1．4％，P＝0．017），CC基因型人群 HDL及载脂蛋白A水平较CT＋ TT基因型高（P＜0．05）。logistic回归分析发现，C242T基因中T等位基因是颈动脉硬化的一个独立危险因素（OR＝1．810，95％ C I：1．454～7．218，P＝0．040）。结论上海松江地区汉族人群C242 T基因多态性与颈动脉硬化相关，且T等位基因可能是颈动脉硬化的一个独立危险因素。
목적：탐토중국상해송강지구한족인군연선알선표령이핵감산린산[NAD （P）H ]양화매 p22phox 아기C242T기인다태성여경동맥경화적상관성。방법선택상해송강지구경동맥경화환자129례작위경동맥경화조，동기건강체검자143례작위대조조，채용병례대조연구，연합취합매련반응‐한제성편단장도다태성분석、기인측서기술，대2조C242T기인다태성위점진행기인형、등위기인빈솔진행분석。결과경동맥경화조C242T기인CT＋TT기인형빈솔급T등위기인빈솔현저고우대조조（10．1％ vs 2．8％，P＝0．028；5．4％ vs 1．4％，P＝0．017），CC기인형인군 HDL급재지단백A수평교CT＋ TT기인형고（P＜0．05）。logistic회귀분석발현，C242T기인중T등위기인시경동맥경화적일개독립위험인소（OR＝1．810，95％ C I：1．454～7．218，P＝0．040）。결론상해송강지구한족인군C242 T기인다태성여경동맥경화상관，차T등위기인가능시경동맥경화적일개독립위험인소。
Objective To study the association between polymorphism of NAD (P ) H oxidase p22phox C242T gene and carotid atherosclerosis in Han population of Shanghai Songjiang Dis‐trict .Methods One hundred and twenty‐nine patients with carotid atherosclerois from Shanghai Songjiang District served as an experimental group and 143 subjects undergoing physical examina‐tion served as a control group in this study .The association between genetypes and allelic gene frequency of C242T gene polymorphism sites was analyzed by PCR‐RFLP and gene sequencing , etc .Results The frequencies of CT + TT genetype and T allelic gene were significantly higher in experimental group than in control group (10 .1% vs 2 .8% ,5 .4% vs 1 .4% ,P<0 .05) .The serum HDL and ApoA levels were significantly higher in patients with CC genetype than in those with CT+ TT genetype (P<0 .05) .That the T allelic gene in C242T was an independent risk factor for carotid atherosclerosis (OR=1 .810 ,95% CI:1 .454-7 .218 ,P=0 .040) .Conclusion Polymor‐phism of C242T is associated with carotid atherosclerosis in Han population of Shanghai Songjiang District .The T allelic gene is an independent risk factor for carotid atherosclerosis .