解放军医药杂志
解放軍醫藥雜誌
해방군의약잡지
Medical & Pharmaceutical Journal of Chinese People's Liberation Army
2015年
10期
100-102
,共3页
陈丽萍%刘雄%夏鹏飞%樊秦%赵磊
陳麗萍%劉雄%夏鵬飛%樊秦%趙磊
진려평%류웅%하붕비%번진%조뢰
当归%多糖%正交设计%分光光度法,紫外线
噹歸%多糖%正交設計%分光光度法,紫外線
당귀%다당%정교설계%분광광도법,자외선
Angelica sinensis%Polysaccharide%Orthogonal design%Spectrophotometry%ultraviolet
目的 优化当归多糖苯酚-硫酸显色法的测定条件. 方法 采用苯酚-硫酸显色法,以对照品葡萄糖的含量为考察指标,检测波长为483 nm,按L9 (34 )正交试验设计筛选最佳显色条件. 结果 浓硫酸和苯酚用量对多糖含量测定的影响较显著,优选出的多糖最佳显色条件为苯酚1. 5 ml、浓硫酸7 ml、反应温度100℃、反应时间为10 min,线性范围为0. 005~0. 02 mg/ml,平均回收率为101. 3%,相对标准偏差为1. 22%. 结论 苯酚硫酸法快速、简便,准确性高、重复性好,可适用于当归多糖的含量测定.
目的 優化噹歸多糖苯酚-硫痠顯色法的測定條件. 方法 採用苯酚-硫痠顯色法,以對照品葡萄糖的含量為攷察指標,檢測波長為483 nm,按L9 (34 )正交試驗設計篩選最佳顯色條件. 結果 濃硫痠和苯酚用量對多糖含量測定的影響較顯著,優選齣的多糖最佳顯色條件為苯酚1. 5 ml、濃硫痠7 ml、反應溫度100℃、反應時間為10 min,線性範圍為0. 005~0. 02 mg/ml,平均迴收率為101. 3%,相對標準偏差為1. 22%. 結論 苯酚硫痠法快速、簡便,準確性高、重複性好,可適用于噹歸多糖的含量測定.
목적 우화당귀다당분분-류산현색법적측정조건. 방법 채용분분-류산현색법,이대조품포도당적함량위고찰지표,검측파장위483 nm,안L9 (34 )정교시험설계사선최가현색조건. 결과 농류산화분분용량대다당함량측정적영향교현저,우선출적다당최가현색조건위분분1. 5 ml、농류산7 ml、반응온도100℃、반응시간위10 min,선성범위위0. 005~0. 02 mg/ml,평균회수솔위101. 3%,상대표준편차위1. 22%. 결론 분분류산법쾌속、간편,준학성고、중복성호,가괄용우당귀다당적함량측정.
Objective To optimize the detection condition of coloration method with phenol- sulphuric acid for polysaccharide in Angelica Sinensis. Methods The glucose content of reference substance was used as a parameter, and the coloration method with phenol-sulphuric acid was used to detect the best coloration method with 483 nm wave length and optimized with the L9 (34 ) orthogonal design. Results The concentrated sulfuric acid and phenol volumes had signifi-cant effects on detecting polysaccharide content. The best coloration method for polysaccharide was 1. 5 ml phenol, 7 ml concentrated sulfuric acid, 100℃ reactive temperature, 10 min reactive time, 0. 005-0. 02 mg/mL linear range, 101. 33%average recovery and 1. 22% RSD (relative standard deviation). Conclusion The phenolsulphuric is rapid, simple and accurate with good repeatability, and can been applied in content detection of polysaccharide in Angelica Sinensis.
