蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
Journal of Bengbu Medical College
2015年
10期
1305-1308
,共4页
急性髓系白血病%JAKs基因%预后
急性髓繫白血病%JAKs基因%預後
급성수계백혈병%JAKs기인%예후
acute myeloid leukemia%JAKs gene%prognosis
目的:分析JAKs家族基因在不同时期急性髓系白血病(AML)细胞中的表达及其与预后的相关性.分析JAKs家族基因在不同时期急性髓系白血病(AML)细胞中的表达及其与预后的相关性.方法:RT-PCR法分别检测AML初治组、复发组、完全缓解组(CR组)以及正常对照组(NC组)骨髓MNC细胞中JAKs家族基因(JAK1、JAK2、JAK3和TYK2 mRNA)的表达.同时对初治组患者随访3年,统计3年生存率.结果:初治组和复发组AML患者JAK3和TYK2 mRNA表达水平均高于CR组和NC组(P<0.05~P<0.01),4组JAK2 mRNA表达水平差异有统计学意义(P<0.05),而初治组和复发组以及CR组和NC组间JAKs家族基因表达水平差异无统计学意义(P>0.05);JAK1、JAK2、JAK3和TYK2阳性组和阴性组间CR率差异无统计学意义(P>0.05).Kaplan-Meier分析显示,JAK1、JAK2、JAK3 和TYK2 mRNA阳性表达组3 年生存率依次为36.75%、33.12%、22.13%和19.07%,而阴性表达者依次为46.56%、41.25%、39.55%和37.02%.COX 风险回归分析显示,JAK3 和TYK2 mRNA阳性表达是影响AML患者预后的独立危险因素(P<0.05).结论:JAKs家族基因在AML发生、发展中发挥重要作用,尤其是JAK3和TYK2 mRNA表达阳性与CR和总体生存率关系密切,直接影响预后.
目的:分析JAKs傢族基因在不同時期急性髓繫白血病(AML)細胞中的錶達及其與預後的相關性.分析JAKs傢族基因在不同時期急性髓繫白血病(AML)細胞中的錶達及其與預後的相關性.方法:RT-PCR法分彆檢測AML初治組、複髮組、完全緩解組(CR組)以及正常對照組(NC組)骨髓MNC細胞中JAKs傢族基因(JAK1、JAK2、JAK3和TYK2 mRNA)的錶達.同時對初治組患者隨訪3年,統計3年生存率.結果:初治組和複髮組AML患者JAK3和TYK2 mRNA錶達水平均高于CR組和NC組(P<0.05~P<0.01),4組JAK2 mRNA錶達水平差異有統計學意義(P<0.05),而初治組和複髮組以及CR組和NC組間JAKs傢族基因錶達水平差異無統計學意義(P>0.05);JAK1、JAK2、JAK3和TYK2暘性組和陰性組間CR率差異無統計學意義(P>0.05).Kaplan-Meier分析顯示,JAK1、JAK2、JAK3 和TYK2 mRNA暘性錶達組3 年生存率依次為36.75%、33.12%、22.13%和19.07%,而陰性錶達者依次為46.56%、41.25%、39.55%和37.02%.COX 風險迴歸分析顯示,JAK3 和TYK2 mRNA暘性錶達是影響AML患者預後的獨立危險因素(P<0.05).結論:JAKs傢族基因在AML髮生、髮展中髮揮重要作用,尤其是JAK3和TYK2 mRNA錶達暘性與CR和總體生存率關繫密切,直接影響預後.
목적:분석JAKs가족기인재불동시기급성수계백혈병(AML)세포중적표체급기여예후적상관성.분석JAKs가족기인재불동시기급성수계백혈병(AML)세포중적표체급기여예후적상관성.방법:RT-PCR법분별검측AML초치조、복발조、완전완해조(CR조)이급정상대조조(NC조)골수MNC세포중JAKs가족기인(JAK1、JAK2、JAK3화TYK2 mRNA)적표체.동시대초치조환자수방3년,통계3년생존솔.결과:초치조화복발조AML환자JAK3화TYK2 mRNA표체수평균고우CR조화NC조(P<0.05~P<0.01),4조JAK2 mRNA표체수평차이유통계학의의(P<0.05),이초치조화복발조이급CR조화NC조간JAKs가족기인표체수평차이무통계학의의(P>0.05);JAK1、JAK2、JAK3화TYK2양성조화음성조간CR솔차이무통계학의의(P>0.05).Kaplan-Meier분석현시,JAK1、JAK2、JAK3 화TYK2 mRNA양성표체조3 년생존솔의차위36.75%、33.12%、22.13%화19.07%,이음성표체자의차위46.56%、41.25%、39.55%화37.02%.COX 풍험회귀분석현시,JAK3 화TYK2 mRNA양성표체시영향AML환자예후적독립위험인소(P<0.05).결론:JAKs가족기인재AML발생、발전중발휘중요작용,우기시JAK3화TYK2 mRNA표체양성여CR화총체생존솔관계밀절,직접영향예후.
Objective:To analyze the expressions of JAKs family-gene in different periods of acute myeloid leukemia( AML) cells,and its correlation with the prognosis of patients. Methods:The mRNA expressions of JAKs family-gene( including JAK1,JAK2,JAK3 and TYK2) in bone marrow MNC of the first-treated group,recurrence group and complete remission group(CR group) and normal control group( NC group) were detected using RT-PCR method. All cases were followed up for 3 years, and the 3-year survival rate was analyzed. Results:The expression levels of the JAK3 and TYK2 mRNA in the first-treated group and recurrence group were higher than those in CR group and NC group(P<0. 05 to P<0. 01),the differences of the expression level of the JAK2 mRNA in the 4 groups were were statisticallg significant(P<0. 05),and the differences of the mRNA expression levels of JAKs family-gene between the first-treated group,recurrence group,CR group and NC group were not statistically significant(P>0. 05). The differences of the CR rate in the JAK1,JAK2,JAK3 and TYK2 positive group and negative group were not statistically significant(P>0. 05). Kaplan-Meier analysis showed that the 3-year survival rates in the JAK1,JAK2,JAK3 and TYK2 mRNA positive expression groups were 36. 75%,33. 12%, 22. 13% and 19. 07%,respectively;the 3-year survival rates in the JAK1,JAK2,JAK3 and TYK2 mRNA negative expression groups were 46. 56%,51. 25%,39. 55% and 37. 02%,respectively. COX regression analysis showed that the positive expressions of JAK3 and TYK2 mRNA were the independent risk factors affecting the prognosis of AML patients. Conclusions:JAKs family genes play an important role in the occurrence and development of AML,especially the positive expressions of JAK3 and TYK2 mRNA are closely connected with CR and overall survival,and directly affect the prognosis.
