CAJ | 학술논문

目的：构建牙髓干细胞－生物蛋白胶支架的三维培养体系，研究牙髓干细胞在生物蛋白胶中的生物相容性。方法体外复苏牙髓干细胞并复合进入生物蛋白胶中培养，通过CCK－8检测提取液对细胞增长情况的影响，并通过细胞的矿化情况进一步分析生物蛋白胶的生物特性。结果冻存的牙髓干细胞经免疫荧光染色具备干细胞的特性，细胞包埋于生物蛋白胶内可见三维培养下细胞状态更加伸展，更加模拟自然生长，细胞形态典型，2 w后生物蛋白胶可完全降解，牙髓干细胞矿化诱导结果较典型。结论生物蛋白胶是一种适宜的组织工程牙髓种子细胞载体。
목적：구건아수간세포－생물단백효지가적삼유배양체계，연구아수간세포재생물단백효중적생물상용성。방법체외복소아수간세포병복합진입생물단백효중배양，통과CCK－8검측제취액대세포증장정황적영향，병통과세포적광화정황진일보분석생물단백효적생물특성。결과동존적아수간세포경면역형광염색구비간세포적특성，세포포매우생물단백효내가견삼유배양하세포상태경가신전，경가모의자연생장，세포형태전형，2 w후생물단백효가완전강해，아수간세포광화유도결과교전형。결론생물단백효시일충괄의적조직공정아수충자세포재체。
Objective To build the three-dimensional cultivation system of dental pulp stem cells with biological fibrin glue bracket , study the compatibility of dental pulp stem cells in biological fibrin glue ,explore the feasibility of the biological fibrin glue in the dental pulp tissue engineering scaffold .Methods The dental pulp stem cells were recovered in vitro and identificated by immunofluorescence staining , and cultivated in biological fibrin glue ,the effect of cell growth was detected by CCK 8,the characteristics of the biological fibrin glue was an-alyzed through mineralization changes of the cells .Results Dental pulp stem cells in frozen storage had the characteristics of stem cells by immunofluorescence staining ,cell state was more stretch ,more simulated natural growth and more the typical forms in embedding in biological fibrin glue,the biological fibrin glue had completely degradations after 2 weeks,induced mineralization results of dental pulp stem cells .Con-clusions Biological fibrin glue is a suitable carrier of dental pulp tissue engineering seed cells .