CAJ | 학술논문

目的:探讨微小RNA(microRNA,miR)-125b及其靶基因信号素分子(SEMA)4C在乳腺癌组织(BRCR)及其癌旁正常组织(NCT)中的表达情况,并分析其与临床病理学特征的关联性及意义.方法:采用荧光定量PCR检测24 例BRCR及其NCT中miR-125b的表达,采用免疫组织化学方法检测40例BRCR及其NCT中SEMA4C的表达.统计分析两者表达水平与患者的年龄、组织学分级、临床分期、淋巴结转移,雌、孕激素受体和人表皮生长因子受体-2(cerbB-2)等临床病理学特征间的关联性.结果:SEMA4C表达在BRCR中高于NCT,SEMA4C表达在淋巴结转移和cerbB-2表达间差异均有统计学意义(P<0.01和P<0.05),而在患者年龄,组织学分级,肿瘤临床分期及雌、孕激素受体表达间差异均无统计学意义(P>0.05).miR-125b表达在BRCR中低于NCT(P<0.05),其表达在SEMA4C、淋巴结转移以及cerbB-2差异均有统计学意义(P=0.034 ~P=0.022).结论:在乳腺浸润性导管癌中SEMA4C表达升高,miR-125b表达降低,两者均与cerbB-2过表达以及淋巴结转移均有一定关系,提示SEMA4C为miR-125b靶基因之一,miR-125b可能是一个新的乳腺癌标志物.
목적:탐토미소RNA(microRNA,miR)-125b급기파기인신호소분자(SEMA)4C재유선암조직(BRCR)급기암방정상조직(NCT)중적표체정황,병분석기여림상병이학특정적관련성급의의.방법:채용형광정량PCR검측24 례BRCR급기NCT중miR-125b적표체,채용면역조직화학방법검측40례BRCR급기NCT중SEMA4C적표체.통계분석량자표체수평여환자적년령、조직학분급、림상분기、림파결전이,자、잉격소수체화인표피생장인자수체-2(cerbB-2)등림상병이학특정간적관련성.결과:SEMA4C표체재BRCR중고우NCT,SEMA4C표체재림파결전이화cerbB-2표체간차이균유통계학의의(P<0.01화P<0.05),이재환자년령,조직학분급,종류림상분기급자、잉격소수체표체간차이균무통계학의의(P>0.05).miR-125b표체재BRCR중저우NCT(P<0.05),기표체재SEMA4C、림파결전이이급cerbB-2차이균유통계학의의(P=0.034 ~P=0.022).결론:재유선침윤성도관암중SEMA4C표체승고,miR-125b표체강저,량자균여cerbB-2과표체이급림파결전이균유일정관계,제시SEMA4C위miR-125b파기인지일,miR-125b가능시일개신적유선암표지물.
Objective:To explore the expressions of microRNA(miR)-125b and its target gene semaphorin4C(SEMA4C) in breast cancer tissue(BRCR) and adjacent normal tissue(NCT),and analyze its correlation with clinicopathologic features and significance. Methods:The expressions of miR-125b in 24 BRCR and NCT were detected using real-time quantitative PCR,and the expressions of SEMA4C in 40 BRCR and NCT were detected by immunohistochemistry. The relationships between the expressions of miR-125b and SEMA4C and clinico-pathological parameters including age,clinical staging,lymph node metastasis,estrogen receptor,progesterone and human epidermal growth factor receptor-2(cerbB-2) were analyzed. Results:The expression of SEMA4C in BRCR was higher than that in NCT,the difference between the expression of SEMA4C and cerbB-2 in lymph node metastasis tissue was statistically significant(P<0. 01 and P<0. 05). The differences of the age,histological grading,clinical staging,and estrogen and progesterone receptors were not statistically significant(P>0. 05). The expression of miR-125b in BRCR was lower than that in NCT(P <0. 05),the differences between the expression of SEMA4C and cerbB-2 in lymph node metastasis tissue were statistically significant ( P =0. 034 to P =0. 022). Conclusions:The expressions of miR-125b and SEMA4C in breast invasive ductal carcinoma increase and decrease, respectively,which is associated with lymph node metastasis and cerbB-2 level. The SEMA4C is one target gene of miR-125b,and miR-125b may serve as a potential breast cancer marker.