湖北农业科学
湖北農業科學
호북농업과학
Hubei Agricultural Sciences
2015年
18期
4441-4445,4463
,共6页
刚果红染料%辣根过氧化物酶%固定化%降解
剛果紅染料%辣根過氧化物酶%固定化%降解
강과홍염료%랄근과양화물매%고정화%강해
Congo red dye%horseradish peroxidase%immobilized%degradation
研究了以固定化的辣根过氧化酶为催化剂,催化H2O2氧化降解刚果红染料,考察了影响刚果红染料降解率的几个重要因素,包括pH﹑温度﹑反应时间﹑H2O2浓度﹑染料初始浓度等,确定其最佳条件为pH 3.5,固定化酶用量0.5 g,H2O2浓度1.0 mmol/L,底物浓度0.1 mmol/L,反应温度40℃,反应时间30 min,降解率达到91.6%. 催化剂重复使用4次后降解产率仍可达到22.0%,同时采用质谱对降解后的产物进行了分析.
研究瞭以固定化的辣根過氧化酶為催化劑,催化H2O2氧化降解剛果紅染料,攷察瞭影響剛果紅染料降解率的幾箇重要因素,包括pH﹑溫度﹑反應時間﹑H2O2濃度﹑染料初始濃度等,確定其最佳條件為pH 3.5,固定化酶用量0.5 g,H2O2濃度1.0 mmol/L,底物濃度0.1 mmol/L,反應溫度40℃,反應時間30 min,降解率達到91.6%. 催化劑重複使用4次後降解產率仍可達到22.0%,同時採用質譜對降解後的產物進行瞭分析.
연구료이고정화적랄근과양화매위최화제,최화H2O2양화강해강과홍염료,고찰료영향강과홍염료강해솔적궤개중요인소,포괄pH﹑온도﹑반응시간﹑H2O2농도﹑염료초시농도등,학정기최가조건위pH 3.5,고정화매용량0.5 g,H2O2농도1.0 mmol/L,저물농도0.1 mmol/L,반응온도40℃,반응시간30 min,강해솔체도91.6%. 최화제중복사용4차후강해산솔잉가체도22.0%,동시채용질보대강해후적산물진행료분석.
Immobilized horseradish peroxidase was used as catalyst of oxidative degradation of Congo red dye by hydrogen per-oxide.Several important factors affecting the degradation rate of Congo red dye, including pH,temperature,reaction time, H2O2 concentration,the initial concentration of the dye etc.were investigated.The obtained optimum conditions are as follows:pH 3.5,0.5 g immobilized enzyme,1.0 mmol/L H2O2,0.1 mmol/L substrate concentration,40℃ reaction temperature and 30 min reac-tion time.Under the optimal conditions the degradation rate could reach to 91.6%.After use of catalyst four times,the rate of degradation rate can still reach to 22.0%.Furthermore,the degradation products were also analyzed by mass spectrometry.
