CAJ | 학술논문

对披针叶黄华(Thermopsis lanceolata)中分离的编号为SP01﹑RM02的2株蠕形分生孢子内生真菌进行种属分类鉴定.观察真菌的形态,提取真菌DNA,扩增并测定其ITS﹑GA PDH序列,构建系统发育树,结合形态特征和遗传进化分析结果,并对其进行种属分类. 结果表明,SP01菌株的形态特征与凸脐孢属(Exserohilum)中的E.rostratum最为相似,与E.rostratum的遗传进化关系最为密切;RM02菌株的形态特征与弯孢属(Curvularia)中C.spicifera、C.australiensis最为相似,与C.spicifera的遗传进化关系最为密切. 确定披针叶黄华中分离的SP01﹑RM02分别为E.rostratum和C.spicifera.
대피침협황화(Thermopsis lanceolata)중분리적편호위SP01﹑RM02적2주연형분생포자내생진균진행충속분류감정.관찰진균적형태,제취진균DNA,확증병측정기ITS﹑GA PDH서렬,구건계통발육수,결합형태특정화유전진화분석결과,병대기진행충속분류. 결과표명,SP01균주적형태특정여철제포속(Exserohilum)중적E.rostratum최위상사,여E.rostratum적유전진화관계최위밀절;RM02균주적형태특정여만포속(Curvularia)중C.spicifera、C.australiensis최위상사,여C.spicifera적유전진화관계최위밀절. 학정피침협황화중분리적SP01﹑RM02분별위E.rostratum화C.spicifera.
This study was conducted to confirm the species of two helminthosporioid fungal endophytes, SP01 and RM02, isolated from wild Thermopsis lanceolata.The two isolates were observed under the light microscope respectively.The DNA of the fungi were extracted for amplifying the fungal ITS and GAPDH sequences that were sequenced subsequently.Homology comparison was conducted and NJ phylogenetic trees were established based on the sequences of the fungi.Two fungal endo-phytes were classified according to the fungal morphology and phylogenetic analysis.The results indicated that SP01 was simi-lar to Exserohilum rostratum.Homology and phylogenetic analyses also showed SP01 to be more closely related to E.rostra-tum.RM02 was more similar to some fungi of Curvularia genus, such as C.spicifera and C.australiensis.Homology and phylogenetic analyses also showed the isolate to be most closely related to C.spicifera.In addition to above results, the two isolates, SP01 and RM02, were classified as E.rostratum and C.spicifera respectively.