CAJ | 학술논문

采用苯胺蓝脱色法和纤维素刚果红培养基鉴别法,从所采样品中分离筛选到8株能较好降解木质纤维素的菌株. 对8个菌株的LiP酶﹑MnP酶﹑Lac酶﹑C1酶﹑Cx酶和纤维二糖酶(BG酶)分别进行测定,得到4株酶类齐全且酶活较高的菌株H14﹑H17﹑W15﹑W23. 在分析菌株相容性基础上进行菌株组合,对菌群的发酵条件进行优化,, 在优化条件下的LiP酶活性可达28.02 U/mL,Cx酶活性达38.16 U/mL,对玉米秸秆粉末的降解率高达35.24%. 该菌群具有进一步研究价值.
채용분알람탈색법화섬유소강과홍배양기감별법,종소채양품중분리사선도8주능교호강해목질섬유소적균주. 대8개균주적LiP매﹑MnP매﹑Lac매﹑C1매﹑Cx매화섬유이당매(BG매)분별진행측정,득도4주매류제전차매활교고적균주H14﹑H17﹑W15﹑W23. 재분석균주상용성기출상진행균주조합,대균군적발효조건진행우화,, 재우화조건하적LiP매활성가체28.02 U/mL,Cx매활성체38.16 U/mL,대옥미갈간분말적강해솔고체35.24%. 해균군구유진일보연구개치.
Using aniline blue decoloration and Congo red medium,8 strains which could degrade the cellulose preferable, were screened from the samples.Lignin peroxidase、Mn peroxidase、Laccase、endoglucanase、exoglucanase and cellobiase activities of 8 strains were analysed, and got 4 strains had total and higher enzymes, that were H14、H17、W15、W23.Based on the analysis of strains consistency,4 strains had been combined.Optimized the fermentation conditions, LiP and Cx activity re-spectively reached 28.02 U/mL and 38.16 U/mL under the optimization conditions, and the degradation rate of corn straw powder reached 35.24%,on the degradation rate of 35.24% corn bran.which had the value of further study.