湖北农业科学
湖北農業科學
호북농업과학
Hubei Agricultural Sciences
2015年
18期
4540-4543
,共4页
胡思玉%彭垠%赵海涛%陈永祥
鬍思玉%彭垠%趙海濤%陳永祥
호사옥%팽은%조해도%진영상
四川裂腹鱼(SchizothoraxkozloviNikolsky)%乳酸脱氢酶%苹果酸脱氢酶%谷氨酸脱氢酶%乙醇脱氢酶%酯酶
四川裂腹魚(SchizothoraxkozloviNikolsky)%乳痠脫氫酶%蘋果痠脫氫酶%穀氨痠脫氫酶%乙醇脫氫酶%酯酶
사천렬복어(SchizothoraxkozloviNikolsky)%유산탈경매%평과산탈경매%곡안산탈경매%을순탈경매%지매
Schizothorax kozlovi%LDH%MDH%GLDH%ADH%EST
采用聚丙烯酰胺凝胶电泳法,对四川裂腹鱼(Schizothorax kozlovi Nikolsky)的眼球﹑心脏﹑肾脏﹑性腺﹑脾脏﹑肝脏﹑脑﹑鳃和肌肉9种组织中的乳酸脱氢酶同工酶﹑苹果酸脱氢酶同工酶﹑谷氨酸脱氢酶同工酶﹑乙醇脱氢酶同工酶﹑酯酶同工酶5种同工酶进行了测定. 结果表明,5种同工酶在四川裂腹鱼9种组织均有表达,且具有明显的组织特异性.
採用聚丙烯酰胺凝膠電泳法,對四川裂腹魚(Schizothorax kozlovi Nikolsky)的眼毬﹑心髒﹑腎髒﹑性腺﹑脾髒﹑肝髒﹑腦﹑鰓和肌肉9種組織中的乳痠脫氫酶同工酶﹑蘋果痠脫氫酶同工酶﹑穀氨痠脫氫酶同工酶﹑乙醇脫氫酶同工酶﹑酯酶同工酶5種同工酶進行瞭測定. 結果錶明,5種同工酶在四川裂腹魚9種組織均有錶達,且具有明顯的組織特異性.
채용취병희선알응효전영법,대사천렬복어(Schizothorax kozlovi Nikolsky)적안구﹑심장﹑신장﹑성선﹑비장﹑간장﹑뇌﹑새화기육9충조직중적유산탈경매동공매﹑평과산탈경매동공매﹑곡안산탈경매동공매﹑을순탈경매동공매﹑지매동공매5충동공매진행료측정. 결과표명,5충동공매재사천렬복어9충조직균유표체,차구유명현적조직특이성.
The expression of five different isoenzymes(lactate dehydrogenase isoenzyme, malate dehydrogenase isoenzyme, glu-tamate dehydrogenase isoenzyme, alcohol dehydrogenase isoenzyme and esterase isoenzyme) in nine different tissues (eye, heart,kidney,gonads,spleen,hepatopancreas,brain,gills and muscle) of Schizothorax kozlovi was examined with polyacrylamide gel electrophoresis (PAGE).It was shown that all five isoenzymes were expressed in nine different tissues and had clearly tissue specificity.