CAJ | 학술논문

目的通过研究电离辐射对小鼠胸腺Th17细胞相关细胞因子的影响,探讨高、低剂量辐射诱导不同的免疫效应中Th17细胞功能.方法将健康ICR小鼠按随机数字表法分为健康对照组、低剂量照射组(0.05、0.075 Gy)和高剂量照射组(0.5、1.0、2.0、4.0 Gy)探讨剂量-效应关系,用X射线深部治疗机进行不同剂量的全身照射,于照射后24 h处死.同时,探讨时间-效应关系,即分为健康对照组、低剂量照射组(0.075 Gy)和高剂量照射组(2.0 Gy),于照射后12、24、48 h处死,取胸腺组织制备成组织匀浆,ELISA法检测小鼠胸腺细胞中白介素-17a(IL-17a)与白介素-21(IL-21)的浓度.结果在时间-效应结果中,与健康对照组相比,0.075 Gy照射组胸腺细胞IL-17a和IL-21分泌量呈下降趋势,其分泌量于48 h降到最低(t=3.85、4.73,P＜0.05);而2.0 Gy照射组均呈大幅度上升趋势,其分泌量在48 h达到最高(t=-6.74、-6.19,P＜0.05);在剂量-效应结果中,与健康对照组相比,较低剂量照射组胸腺细胞IL-17a与IL-21分泌量下降,在0.05 Gy最低(=8.39、16.45,P＜0.05);较高剂量照射组胸腺细胞的分泌量上升,在4.0 Gy时升至最高(t=-15.60、-18.62,P＜0.05).结论高剂量辐射可以诱导小鼠胸腺细胞IL-17a与IL-21分泌量的增加,而低剂量辐射使其下降,表明Th17细胞相关的细胞因子在低剂量辐射诱导的免疫功能增强效应和高剂量辐射诱导免疫抑制效应中起着重要的作用.
목적 통과연구전리복사대소서흉선Th17세포상관세포인자적영향,탐토고、저제량복사유도불동적면역효응중Th17세포공능.방법 장건강ICR소서안수궤수자표법분위건강대조조、저제량조사조(0.05、0.075 Gy)화고제량조사조(0.5、1.0、2.0、4.0 Gy)탐토제량-효응관계,용X사선심부치료궤진행불동제량적전신조사,우조사후24 h처사.동시,탐토시간-효응관계,즉분위건강대조조、저제량조사조(0.075 Gy)화고제량조사조(2.0 Gy),우조사후12、24、48 h처사,취흉선조직제비성조직균장,ELISA법검측소서흉선세포중백개소-17a(IL-17a)여백개소-21(IL-21)적농도.결과 재시간-효응결과중,여건강대조조상비,0.075 Gy조사조흉선세포IL-17a화IL-21분비량정하강추세,기분비량우48 h강도최저(t=3.85、4.73,P＜0.05);이2.0 Gy조사조균정대폭도상승추세,기분비량재48 h체도최고(t=-6.74、-6.19,P＜0.05);재제량-효응결과중,여건강대조조상비,교저제량조사조흉선세포IL-17a여IL-21분비량하강,재0.05 Gy최저(=8.39、16.45,P＜0.05);교고제량조사조흉선세포적분비량상승,재4.0 Gy시승지최고(t=-15.60、-18.62,P＜0.05).결론 고제량복사가이유도소서흉선세포IL-17a여IL-21분비량적증가,이저제량복사사기하강,표명Th17세포상관적세포인자재저제량복사유도적면역공능증강효응화고제량복사유도면역억제효응중기착중요적작용.
Objective To study the cytokine releases in the thymus Th17 cells of irradiated mice, and to explore the function of Th17 cells in different immune responses to radiation of different doses.Methods According to the random number table method, ICR mice were divided into three groups i.e., healthy control group, low-dose group (0.05, 0.075 Gy) and high-dose group (0.5, 1.0, 2.0, 4.0 Gy) to explore the dose-effect relationship.In the dose-effect group, the ICR mice exposed to different dosages of whole body X-ray irradition were decapitated at 24 h after irradiation.Meanwhile, ICR mice were divided into three groups i.e., healthy control group, low-dose group (0.075 Gy) and high-dose group (2.0 Gy) to explore the time-effect relationship.The mice were decapitated after 12, 24, and 48 h of 0.075 and 2 Gy of whole body X-ray irradiation, then prepared for the thymus tissue homogenization.The expressions of IL-17a and IL-21 in the thymus tissue homogenization were detected by ELISA.Results For the time-effect, following a low dose irradiation of 0.075 Gy, the levels of IL-17a and IL-21 in the thymocytes continuously decreased along with the time post-irradiation and reached its lowest value at 48 h after radiation but it was still higher than that in the control group (t =3.85, 4.73, P ＜ 0.05);the expressions of these cytokines dramatically increased along with the time post-irradiation and reached to its highest level at 48 h after irradiation of 2.0 Gy (t =-6.74,-6.19, P＜0.05).For the dose-effect group, at 24 h after irradiation, the expressions of IL-17a and IL-21 in mouse thymocytes were lower than controls for low dose groups and had the lowest level at 0.05 Gy(t =8.39, 16.45, P ＜ 0.05) , while they increased in the high dose groups and had the highest level at 4.0 Gy(t =-15.60,-18.62, P ＜ 0.05).Conclusions After irradiation, the secretions of IL-17a and IL-21 in mouse thymocytes are inhibited by low dose irradiation but triggered by higher doses, which indicates that Th17-related cytokines play an important role in immune suppression induced by a high dose radiation, while the immune function enhancement is induced by low dose radiation.