CAJ | 학술논문

目的:研究4-氨基吡啶(4-AP)对人舌鳞状细胞癌(OTSCC)Tca8113细胞体外迁徙和侵袭能力的影响.方法: 采用划痕愈合方法及Transwell侵袭实验检测10、20、30 mmol/L 4-AP干预Tca8113细胞后对Tca8113细胞迁徙及侵袭能力的影响.结果:浓度分别为10 mmol/L、20 mmol/L、30 mmol/L 4-AP干预Tca8113细胞后,Tca8113细胞12 h迁徙率分别为(14.07±5.59)%、(11.34±1.79)%及(8.71±3.26)%;24 h的迁徙率分别为(22.20±5.30)%、(14.24± 2.52)%及(11.65±2.97)%,均明显小于对照组(19.25±4.50)%和(33.16±6.40)%,差异具有显著性(P<0.01). 细胞侵袭实验结果显示,10 mmol/L、20 mmol/L、30 mmol/L的4-AP作用于Tca8113细胞24 h后穿膜细胞数分别为(112.20± 24.36)、(28.87±6.47)、(11.80±5.77)个,随着4-AP浓度的升高,Tca8113穿膜细胞数明显下降,与对照组(179.73±16.14)相比,差异具有显著性(P<0.001). 结论:4-AP具有抑制Tca8113舌鳞癌细胞株体外迁徙侵袭的能力.
목적:연구4-안기필정(4-AP)대인설린상세포암(OTSCC)Tca8113세포체외천사화침습능력적영향.방법: 채용화흔유합방법급Transwell침습실험검측10、20、30 mmol/L 4-AP간예Tca8113세포후대Tca8113세포천사급침습능력적영향.결과:농도분별위10 mmol/L、20 mmol/L、30 mmol/L 4-AP간예Tca8113세포후,Tca8113세포12 h천사솔분별위(14.07±5.59)%、(11.34±1.79)%급(8.71±3.26)%;24 h적천사솔분별위(22.20±5.30)%、(14.24± 2.52)%급(11.65±2.97)%,균명현소우대조조(19.25±4.50)%화(33.16±6.40)%,차이구유현저성(P<0.01). 세포침습실험결과현시,10 mmol/L、20 mmol/L、30 mmol/L적4-AP작용우Tca8113세포24 h후천막세포수분별위(112.20± 24.36)、(28.87±6.47)、(11.80±5.77)개,수착4-AP농도적승고,Tca8113천막세포수명현하강,여대조조(179.73±16.14)상비,차이구유현저성(P<0.001). 결론:4-AP구유억제Tca8113설린암세포주체외천사침습적능력.
Objective:To explore the effect of 4-AP on the migration and invasion of Tca8113 cells,thus provide reference for clinical studies. Method:Wound healing assay and Transwell assay were employed to explore whether 4-AP (10,20,30 mmol/L)could inhibit the migration and invasion of Tca8113 cell. Result:①The scratch healing assay obtained cell migration rates of (19.25±4.50)%,(14.07±5.59)%,(11.34±1.79)%and (8.71±3.26)%for Tca8113 cells treated with 10,20,30 mmol/L of 4-AP for 12 h while(33.16±6.40)%,(22.20±5.30)%,(14.24±2.52)%and(11.65±2.97)%for 24 h. The results showed that the migration rates were significantly difference between 4-AP goups (10,20,30 mmol/L)and control groups (P <0.01). ②The transwell invasion assay showed that with the increasing of 4-AP concentration,the Tca8113 cell number decrased. The number of cells permeating through the matrigel were (112.20±24.36),(28.87±6.47), (11.80±5.77) when the Tca8113 cells were treated with 10 mmol/L,20 mmol/L,30 mmol/L 4-AP for 24 h,while the control group was (179.73 ±16.14),and the difference was significant (F=392.045,P <0.001). Conclusion:4-AP can significantly inhibited the migration and invasion of human tongue carcinoma cell line Tca8113.