CAJ | 학술논문

通过生物信息学方法对水稻(Oryza sativa L.)蜡质合成相关基因OsCER4启动子序列和表达特性进行了分析;通过构建OsCER4启动子驱动的GUS融合表达载体,分析了OsCER4基因的时空表达;并分别以200 mmol/L NaCl﹑100μmol/L ABA和1.0% H2O2处理水稻幼苗, 通过半定量RT-PCR分析逆境胁迫下OsCER4的表达模式. 启动子序列分析表明,OsCER4启动子中包括大量根﹑茎﹑叶肉等特异表达位点以及与干旱﹑冷﹑盐等多种逆境胁迫相关的调控序列.表达特征分析表明,OsCER4基因在愈伤组织﹑根﹑茎﹑叶中均有表达,在颖壳和子房中也有表达. NaCl和PEG等逆境胁迫下,OsCER4基因表达量在不同处理时间有所增加,H2O2胁迫下,OsCER4基因表达量有所下降.
통과생물신식학방법대수도(Oryza sativa L.)사질합성상관기인OsCER4계동자서렬화표체특성진행료분석;통과구건OsCER4계동자구동적GUS융합표체재체,분석료OsCER4기인적시공표체;병분별이200 mmol/L NaCl﹑100μmol/L ABA화1.0% H2O2처리수도유묘, 통과반정량RT-PCR분석역경협박하OsCER4적표체모식. 계동자서렬분석표명,OsCER4계동자중포괄대량근﹑경﹑협육등특이표체위점이급여간한﹑랭﹑염등다충역경협박상관적조공서렬.표체특정분석표명,OsCER4기인재유상조직﹑근﹑경﹑협중균유표체,재영각화자방중야유표체. NaCl화PEG등역경협박하,OsCER4기인표체량재불동처리시간유소증가,H2O2협박하,OsCER4기인표체량유소하강.
In this study, the promoter and spatial expression of wax biosynthesis related gene OsCER4 in rice(Oryza sativa L.) was analyzed via bioinformatics,and the temporal and spatial expression of OsCER4 was analyzed by GUS histochemical stain-ing.In addition, the expression of OsCER4 was analyzed by semi-quantitative RT-PCR when the seedlings were treated with stresses, including 200 mmol/L NaCl,100μmol/L ABA and 1.0% H2O2.The bioinformatics analysis showed that the promoter of OsCER4 gene (about 2 kb upstream of OsCER4) was predicted to contain important regulatory elements including drought, high salt, coldness,ABA,light regulation,copper,and oxygen responsive elements.The analysis of OsCER4 gene expression profiles re-vealed that OsCER4 was expressed in callus,roots,stems,leaves as well as glume and ovary.The analysis of OsCER4 gene in response to stresses showed that the expression of OsCER4 gene increased at different time when the seedlings were treated with NaCl and PEG, however, the expression of OsCER4 gene in seedlings decreased when treated with H2O2.