CAJ | 학술논문

黑曲霉发酵是工业生产柠檬酸的主要途径，研究该过程的动力学模型对更好地理解这一工业微生物的代谢机理有重要意义。基于黑曲霉（Aspergillus niger）柠檬酸发酵过程主要代谢途径的分析，构建了简化的碳代谢网络，建立了胞内物质和酶浓度的平衡模型。引入一阶闭环调节器模型描述发酵初期微生物酶系的建立过程。结合生物反应器模型，给出了重要宏观状态变量（如细胞物质浓度、基质浓度、产物浓度）和胞内酶浓度的动态仿真结果与实验值的对比。
흑곡매발효시공업생산저몽산적주요도경，연구해과정적동역학모형대경호지리해저일공업미생물적대사궤리유중요의의。기우흑곡매（Aspergillus niger）저몽산발효과정주요대사도경적분석，구건료간화적탄대사망락，건립료포내물질화매농도적평형모형。인입일계폐배조절기모형묘술발효초기미생물매계적건립과정。결합생물반응기모형，급출료중요굉관상태변량（여세포물질농도、기질농도、산물농도）화포내매농도적동태방진결과여실험치적대비。
Aspergillus niger fermentation is the main way for the industrial production of citric acid. Modeling of Aspergillus niger from the perspective of cybernetics is significant for better understanding the metabolic behavior of this industrial microorganism. The metabolic pathways inAspergillusniger during citric acid fermentation are investigated. Based on the simplified metabolic network, a cybernetic model describing the balance of intracellular metabolites and enzyme levels is constructed. Meanwhile, a first-order closed-loop regulator model is used to describe the transient enzyme pool establishment shortly after the inoculation. Combining with the bioreactor model, it is found that model simulations of intracellular enzyme concentrations are in coincidence with the measurements qualitatively, while the macroscopic state variables (biomass concentration, substrate concentrate and product concentrations) agree well with the offline assays.