CAJ | 학술논문

为研究胆酸对营养性肥胖模型大鼠脂肪代谢的影响，本试验将80只雌雄各半的大鼠随机分为5组，每组4个重复，每个重复4只。阴性对照组饲喂脂肪含量为5%的基础饲料，高脂模型组、低剂量组、中剂量组、高剂量组饲喂脂肪含量为35%的高脂饲料，其中低、中、高剂量组分别灌胃16.7、50、150 mg/kg 胆酸，各组连续给药17 d 后，分别测定每组大鼠增重率、Lee’s 指数、血清总胆固醇、血清总甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇等指标。结果表明：模型组、低剂量组及中剂量组大鼠增重率分别较阴性对照组提高39.89%、37.7%、44.9%（P ＜0.01）。高剂量组增重率与阴性对照组无显著差异（P ＞0.05），较模型组降低31.81%（P ＜0.01）。低剂量组及高剂量组的Lee’s 指数分别较模型组降低2.78%和2.77%（P ＜0.05）。高剂量组WAT 湿重、脂肪系数和低密度脂蛋白胆固醇水平分别较模型组降低25.35%（P ＜0.01）、20.42%（P ＜0.05）和20.3%（P ＜0.05）。综上所述，高剂量的胆酸对营养性肥胖大鼠有较好的减肥作用。
위연구담산대영양성비반모형대서지방대사적영향，본시험장80지자웅각반적대서수궤분위5조，매조4개중복，매개중복4지。음성대조조사위지방함량위5%적기출사료，고지모형조、저제량조、중제량조、고제량조사위지방함량위35%적고지사료，기중저、중、고제량조분별관위16.7、50、150 mg/kg 담산，각조련속급약17 d 후，분별측정매조대서증중솔、Lee’s 지수、혈청총담고순、혈청총감유삼지、고밀도지단백담고순、저밀도지단백담고순등지표。결과표명：모형조、저제량조급중제량조대서증중솔분별교음성대조조제고39.89%、37.7%、44.9%（P ＜0.01）。고제량조증중솔여음성대조조무현저차이（P ＞0.05），교모형조강저31.81%（P ＜0.01）。저제량조급고제량조적Lee’s 지수분별교모형조강저2.78%화2.77%（P ＜0.05）。고제량조WAT 습중、지방계수화저밀도지단백담고순수평분별교모형조강저25.35%（P ＜0.01）、20.42%（P ＜0.05）화20.3%（P ＜0.05）。종상소술，고제량적담산대영양성비반대서유교호적감비작용。
This study was to investigate effect of cholic acid (CA) on the lipid mobilization of diet-reduced obesity model rats.A total of 80 rats (half male and half female) were randomly divided into 5 groups with 4 replicates of 4 each group.The control group was fed normal diet contained 5% fat,the model group,low dose group,middle dose group and high dose group was fed high fat diet contained 35% fat,and low dose group,middle dose group,high dose group was ad-ministrated of CA 16.7,50,150 mg/kg.Changes of body weight gain,Lee’s index and biochemical indices were measured after oral administration of CA for 17 d.The results showed that:the weight gain rate of rats were significantly augmented by 39.89%,37.7%,44.9% in the model group,low dose group and middle dose group,compared with control group (P <0.01),but no significance changes was found in the high dose group(P>0.05).The body weight gain was significantly re-duced by 31.81% in high dose group,compared with model group (P < 0.01).the Lee’s index were significantly reduced by 2.78%and 2.77%in low dose group and high dose group,compared with model group (P<0.05);the weight of white adipose tissue(WAT),the adipose coefficient and the level of low density lipoprotein cholesterol(LDL-C) were significant-ly reduced by 25.35%(P < 0.01),20.42%(P < 0.05),20.3%(P < 0.05),compared with model group.Therefore,the high dose CA had relatively obviously function of anti-obesity on diet-reduced obesity model rats.