CAJ | 학술논문

目的：建立肿瘤细胞内2-甲氧基雌二醇含量的测定方法，并测定不同时间、不同细胞系对2-甲氧基雌二醇的吸收情况。方法给予肿瘤细胞含有2-甲氧基雌二醇的培养基，固定时间点收集细胞，细胞破碎液进行液-液萃取，采用反相高效液相色谱法测定2-甲氧基雌二醇含量。结果所选择的4种肿瘤细胞系最高吸收值均在2 h，不同细胞系对药物的吸收量略有差别。结论该方法准确、操作简单、灵敏度高，适合用于肿瘤细胞内2-甲氧基雌二醇含量的测定。
목적：건립종류세포내2-갑양기자이순함량적측정방법，병측정불동시간、불동세포계대2-갑양기자이순적흡수정황。방법급여종류세포함유2-갑양기자이순적배양기，고정시간점수집세포，세포파쇄액진행액-액췌취，채용반상고효액상색보법측정2-갑양기자이순함량。결과소선택적4충종류세포계최고흡수치균재2 h，불동세포계대약물적흡수량략유차별。결론해방법준학、조작간단、령민도고，괄합용우종류세포내2-갑양기자이순함량적측정。
Objective To establish a method for determination the 2 -methoxyestradiol in cells,and to measure the absorption of 2-methoxyestradiol during different times,in different cell lines. Methods The medium contai-ning 2-methoxyestradiol were given into cells,the cells were collected at fixed times,cell lysate was used to liquid-liquid extraction. Reversed-phase high performance liquid chromatography method was used to determine the cont-ention of 2-methoxyestradiol. Results Highest absorption value of the four selected cell lines was 2 h,the drug up-take in different cell lines was slightly different. Conclusion The method is accurate,simple operation,high sen-sitivity,suitable for the determination of 2-methoxyestradiol contention in tumor cell lines.