大连医科大学学报
大連醫科大學學報
대련의과대학학보
Journal of Dalian Medical University
2015年
5期
425-428
,共4页
季英楠%阿良%赵忠海%邓纯博
季英楠%阿良%趙忠海%鄧純博
계영남%아량%조충해%산순박
脊髓损伤%血清β-淀粉样前体蛋白%超敏C反应蛋白
脊髓損傷%血清β-澱粉樣前體蛋白%超敏C反應蛋白
척수손상%혈청β-정분양전체단백%초민C반응단백
spinal cord injury%β-amyloid precursor protein%hypersensitive-cross-reactive protein
目的:探讨大鼠急性脊髓损伤( SCI )程度与血清β-淀粉样前体蛋白(β-APP )和超敏C反应蛋白( hs-CRP)含量变化的相关性。方法取雌性SD大鼠90只,采用动脉夹压力法制备大鼠脊髓轻度损伤(轻度损伤组,30只)和重度损伤(重度损伤组,30只)动物模型,另30只大鼠仅行椎板切除作为假手术组。通过行为学观察( BBB评分)了解脊髓损伤程度及变化,分别于造模成功后12 h、24 h、3 d、7 d、14 d采股静脉血,ELISA检测不同程度SCI大鼠伤后血清β-APP、hs-CRP含量变化。结果重度脊髓损伤组与轻度损伤、假手术组各时间点BBB评分差异有显著性意义( P<0.05)。β-APP于损伤后24 h达到高峰后逐渐回落,hs-CRP于3 d达到高峰后回落。重度脊髓损伤组与轻度脊髓损伤、假手术组各时间点β-APP、hs-CRP比较差异均有显著性意义( P<0.05);轻度脊髓损伤组与假手术组β-APP、hs-CRP水平除14 d点差异无显著性意义外,其余各时间点差异均有显著性意义(P<0.05)。结论急性SCI大鼠血清β-APP、hs-CRP水平变化可反映SCI的严重程度,β-APP比hs-CRP的变化敏感。
目的:探討大鼠急性脊髓損傷( SCI )程度與血清β-澱粉樣前體蛋白(β-APP )和超敏C反應蛋白( hs-CRP)含量變化的相關性。方法取雌性SD大鼠90隻,採用動脈夾壓力法製備大鼠脊髓輕度損傷(輕度損傷組,30隻)和重度損傷(重度損傷組,30隻)動物模型,另30隻大鼠僅行椎闆切除作為假手術組。通過行為學觀察( BBB評分)瞭解脊髓損傷程度及變化,分彆于造模成功後12 h、24 h、3 d、7 d、14 d採股靜脈血,ELISA檢測不同程度SCI大鼠傷後血清β-APP、hs-CRP含量變化。結果重度脊髓損傷組與輕度損傷、假手術組各時間點BBB評分差異有顯著性意義( P<0.05)。β-APP于損傷後24 h達到高峰後逐漸迴落,hs-CRP于3 d達到高峰後迴落。重度脊髓損傷組與輕度脊髓損傷、假手術組各時間點β-APP、hs-CRP比較差異均有顯著性意義( P<0.05);輕度脊髓損傷組與假手術組β-APP、hs-CRP水平除14 d點差異無顯著性意義外,其餘各時間點差異均有顯著性意義(P<0.05)。結論急性SCI大鼠血清β-APP、hs-CRP水平變化可反映SCI的嚴重程度,β-APP比hs-CRP的變化敏感。
목적:탐토대서급성척수손상( SCI )정도여혈청β-정분양전체단백(β-APP )화초민C반응단백( hs-CRP)함량변화적상관성。방법취자성SD대서90지,채용동맥협압역법제비대서척수경도손상(경도손상조,30지)화중도손상(중도손상조,30지)동물모형,령30지대서부행추판절제작위가수술조。통과행위학관찰( BBB평분)료해척수손상정도급변화,분별우조모성공후12 h、24 h、3 d、7 d、14 d채고정맥혈,ELISA검측불동정도SCI대서상후혈청β-APP、hs-CRP함량변화。결과중도척수손상조여경도손상、가수술조각시간점BBB평분차이유현저성의의( P<0.05)。β-APP우손상후24 h체도고봉후축점회락,hs-CRP우3 d체도고봉후회락。중도척수손상조여경도척수손상、가수술조각시간점β-APP、hs-CRP비교차이균유현저성의의( P<0.05);경도척수손상조여가수술조β-APP、hs-CRP수평제14 d점차이무현저성의의외,기여각시간점차이균유현저성의의(P<0.05)。결론급성SCI대서혈청β-APP、hs-CRP수평변화가반영SCI적엄중정도,β-APP비hs-CRP적변화민감。
Objective To investigate the relation to the degree of the acute spinal cord injury ( SCI) by detecting the serumβ-amyloid precursor protein (β-APP) and hypersensitive-cross-reactive protein ( hs-CRP) levels after acute spinal cord injury in rate at different time points.Methods Ninety healthy adult female SD rats were randomly assigned into 3 groups.The bulldog clamp was used to create models of severe SCI ( group A) and mild SCI ( group B) .In group C, only laminectomy was performed without SCI.The ethological study ( BBB scores) was used to learn the degree of the acute spi-nal cord injury.The serum was collected at 12 h, 24 h, 3 d, 7 d, and 14 d after operation through femoral vein.Serumβ-APP and hs-CRP were detected by ELISA.Results The serumβ-APP and hs-CRP reached the peak at 24 h and 3 d, respectively after acute SCI.Serumβ-APP and hs-CRP levels in SCI groups ( A and B) were both significantly higher than those in without SCI group ( C) at all time points ( P<0.05) , except at 14 d between group B and group C. Conclusions The serumβ-APP and hs-CRP levels are positively associated with the severity of SCI.β-APP would be a more sensitive indicator than hs-CRP in a certain time.A acute SCI model of SD rats can be established by bulldog clamp, in order to mimic the clinical SCI.