CAJ | 학술논문

目的：建立稳定的裸鼠结直肠癌肝转移半脾模型。方法利用裸鼠脾脏的特殊解剖，建成半脾模型后，将人HT29结直肠癌细胞接种于近端半脾被膜下，远端半脾埋入皮下备治疗所需。采用不同浓度的肿瘤细胞（5×105／100μL、1×106／100μL及5×106／100μL）或不同浓度的环磷酰胺（ L－CTX和H－CTX），共分6组观察制模30天后的肝转移评分、淋巴结转移及血性腹水情况。结果人HT29结直肠癌细胞建立的肝转移半脾模型成瘤率和减瘤率皆为100％。 L－CTX治疗组和H－CTX治疗组观察结果，发现随结直肠癌细胞浓度梯度的增高肝转移评分也增高，其不同浓度梯度间的差异有显著性意义（两组均为P＜0．05）。 L－CTX治疗组淋巴结转移和血性腹水明显少于H－CTX治疗组，其差异也有显著性意义（P＜0．05）。人HT29结直肠癌细胞浓度梯度为1×106／100μL制模组，显示其模型稳定性和抗肿瘤药物的敏感性最好。结论裸鼠结直肠癌肝转移半脾模型为结直肠癌肝转移的生物学机制和抗转移治疗提供了较为理想的实验模型。肝转移半脾模型为抗肿瘤药物体外筛选模型方法，其模型的建立能应用于抗肿瘤药物的筛选。
목적：건립은정적라서결직장암간전이반비모형。방법이용라서비장적특수해부，건성반비모형후，장인HT29결직장암세포접충우근단반비피막하，원단반비매입피하비치료소수。채용불동농도적종류세포（5×105／100μL、1×106／100μL급5×106／100μL）혹불동농도적배린선알（ L－CTX화H－CTX），공분6조관찰제모30천후적간전이평분、림파결전이급혈성복수정황。결과인HT29결직장암세포건립적간전이반비모형성류솔화감류솔개위100％。 L－CTX치료조화H－CTX치료조관찰결과，발현수결직장암세포농도제도적증고간전이평분야증고，기불동농도제도간적차이유현저성의의（량조균위P＜0．05）。 L－CTX치료조림파결전이화혈성복수명현소우H－CTX치료조，기차이야유현저성의의（P＜0．05）。인HT29결직장암세포농도제도위1×106／100μL제모조，현시기모형은정성화항종류약물적민감성최호。결론라서결직장암간전이반비모형위결직장암간전이적생물학궤제화항전이치료제공료교위이상적실험모형。간전이반비모형위항종류약물체외사선모형방법，기모형적건립능응용우항종류약물적사선。
Objective To establish a feasible and stable hepatic metastases of colon cancer model with nude mice hemis-pleen method.Methods The spleen was divided, creating two hemispleens with their own vascular pedicle.HT29 human colon cancer cells were injected beneath the inferior hemispleen capsules of nude mice, establishing models of liver metas-tases in nude mice.Then removing the inferior hemispleen, the superior hemispleen was placed in the left s.c.pocket for treatmentinjection.The liver metastasis score, lymph node metastasis, and bloody ascites were studied after different con-centrations of HT29 human colon cancer cells infection (5 ×105/100 μL, 1 ×106/100 μL and 5 ×106/100 μL) and dif-ferent concentrations of cyclophosphamide ( CTX) therapy ( L-CTX and H-CTX) .Results The rates of tumor formation and tumor reduce were all 100% in the hepatic metastases of colon cancer hemispleen models.The hepatic metastasis scores in the low concentration CTX group ( L-CTX group) and high concentration CTX group ( H-CTX group) both in-creased when increased theconcentrations of HT29 human colon cancer cells injection (P<0.05).The mice with lymph node metastases or bloody ascites were significantly fewer in L-CTX group than in H -CTX group (P <0.05).The hemispleen model with infection 1 ×106/100μL HT29 human colon cancer cells showed better in model stability.Conclu-sion The hemispleen model are technically feasible and reproducible in researche on the metastatic mechanism and thera-peutic development of colorectal cancer.The feasible and stable hemispleen model could be used in screening antitumor drugs.