CAJ | 학술논문

目的：探讨MICA基因第5外显子微卫星多态性与卵巢癌发病的关系。方法实验组包括95名临床已确诊卵巢癌患者，对照组包括99名无血缘关系的辽宁汉族健康女性。采用PCR－毛细管电泳方法检测被试MICA基因第5外显子微卫星的基因型。结果两组均检测出5种等位基因，分别为A4（179 bp ）、A5（182 bp ）、A5．1（183 bp）、A6（185 bp）和A9（194 bp）。正常对照组MICA第5外显子A5．1等位基因的频率最高，为33．8％。实验组和对照组MICA第5外显子A5等位基因的频率分别为31．1％和19．2％，差异有显著性意义（P＜0．01）。结论 MICA基因第5外显子A5等位基因增加了卵巢癌的发病风险。
목적：탐토MICA기인제5외현자미위성다태성여란소암발병적관계。방법실험조포괄95명림상이학진란소암환자，대조조포괄99명무혈연관계적료녕한족건강녀성。채용PCR－모세관전영방법검측피시MICA기인제5외현자미위성적기인형。결과량조균검측출5충등위기인，분별위A4（179 bp ）、A5（182 bp ）、A5．1（183 bp）、A6（185 bp）화A9（194 bp）。정상대조조MICA제5외현자A5．1등위기인적빈솔최고，위33．8％。실험조화대조조MICA제5외현자A5등위기인적빈솔분별위31．1％화19．2％，차이유현저성의의（P＜0．01）。결론 MICA기인제5외현자A5등위기인증가료란소암적발병풍험。
Objective To explore the relationship between microsatellite polymorphism of MICA gene exon 5 and the risk of ovarian cancer.Methods A total of 95 ovarian cancer patients and 99 unrelated healthy people were recruited in this study.PCR-capillary electrophoresis method was used to detect the microsatellite genotypes of MICA gene exon 5.Re-sults Five alleles were observed in both groups, A4(179 bp),A5(182 bp),A5.1(183 bp), A6(185 bp)and A9(194 bp), respectively.In the control group, A5.1 was the most common allele (33.8%).The frequency of A5 allele of MICA gene in ovarian cancer patients was significantly higher than that in controls (31.1%vs.19.2%, P<0.01).Conclusion A5 allele of MICA gene exon 5 could be associated with high risk of ovarian cancer.