CAJ | 학술논문

目的：通过比较和分析梅毒螺旋体四种血清学检测方法的敏感性及特异性，寻找出梅毒螺旋体抗体合理的检测模式。方法应用甲苯胺红不加热血清试验(TRUST)、梅毒螺旋体酶联免疫吸附试验(TP-ELISA)、梅毒胶体金试验(SYP)及梅毒螺旋体抗体明胶颗粒凝集试验(TPPA)对我院183例临床确诊病例及217例健康体检者的血清标本进行检测，比较四种检测方法敏感性及特异性是否存在差异。结果 ELISA、SYP 和TPPA 三种方法的敏感性均很高，分别为96.7%、99.4%和98.9%，三者之间差异无统计学意义(P＞0.05)，ELISA、SYP和TPPA三种方法的敏感性均高于TRUST(P＜0.05)；四种方法的特异性均很高，分别为97.7%、98.6%、99.5%和98.6%，四者之间差异均无统计学意义(P＞0.05)。结论结合各方法优缺点，探索出先用TP-ELISA初筛，阳性标本同时采用SYP比对，如两种定性方法结果不符，再用TPPA确诊，阳性标本最后用TRUST检测非特异性抗性滴度的合理检测模式。
목적：통과비교화분석매독라선체사충혈청학검측방법적민감성급특이성，심조출매독라선체항체합리적검측모식。방법응용갑분알홍불가열혈청시험(TRUST)、매독라선체매련면역흡부시험(TP-ELISA)、매독효체금시험(SYP)급매독라선체항체명효과립응집시험(TPPA)대아원183례림상학진병례급217례건강체검자적혈청표본진행검측，비교사충검측방법민감성급특이성시부존재차이。결과 ELISA、SYP 화TPPA 삼충방법적민감성균흔고，분별위96.7%、99.4%화98.9%，삼자지간차이무통계학의의(P＞0.05)，ELISA、SYP화TPPA삼충방법적민감성균고우TRUST(P＜0.05)；사충방법적특이성균흔고，분별위97.7%、98.6%、99.5%화98.6%，사자지간차이균무통계학의의(P＞0.05)。결론결합각방법우결점，탐색출선용TP-ELISA초사，양성표본동시채용SYP비대，여량충정성방법결과불부，재용TPPA학진，양성표본최후용TRUST검측비특이성항성적도적합리검측모식。
Objective By comparing and analyzing the sensitivities and specificities of four kinds of syphilis serology detec-tion methods, to find out a reasonable detection model of the Treponema pallidum antibody. Methods Serum samples from 183 patients diagnosed with syphilis and 217 healthy individuals in our hospital were tested by toluidine red unheated serum test (TRUST), Treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA), syphilis colloidal gold test (SYP) and Treponema pallidum particle assay (TPPA). Then the sensitivities and specificities of the four methods were compared. Results The sensitivi-ties of ELISA, SYP and TPPA were high, which were 96.7%, 99.4%and 98.9%respectively and had no significant difference (P>0.05). The sensitivities of ELISA, SYP, TPPA were higher than that of TRUST (P<0.05). The specificities of ELISA, SYP, TPPA and TRUST were 97.7%, 98.6%, 99.5% and 98.6% respectively, which had no significant difference (P>0.05). Conclusion Ac-cording to the advantages and disadvantages of the four methods, it is a reasonable detection model that screening positive samples by TP-ELISA and the positive samples are simultaneously detected by SYP. If the results of these two qualitative methods do not match, and then confirmed by TPPA. Finally, the non-specific antibody titer of the positive samples are detected by TRUST.