CAJ | 학술논문

目的：探讨卵巢早衰大鼠卵巢中原癌基因c-erbB2和EGFR的表达。方法20只有动情周期的雌性大鼠随机分为空白组和实验组。实验组大鼠口服雷公藤多甙片悬浮液,按照50mg/Kg.d，共14d,造POF模型；空白组大鼠同剂量生理盐水灌胃，1次/d，共14d。给药结束后24h,空白组和实验组大鼠取血标本后处死，留取卵巢。留取的卵巢制切片后行HE染色和免疫组化。结果卵巢HE染色发现空白组卵泡生长活跃,可见较多的成熟和次级卵泡,而实验组卵巢次级和成熟卵泡明显减少(P<0.01)，可见较多闭锁卵泡(P<0.01)，卵泡的颗粒细胞层次明显减少；免疫组化发现空白组ErbB2蛋白和EGFR蛋白阳性信号明显，主要位于卵母细胞胞膜上，为深棕色染色颗粒，实验组则阳性表达较少。结论 c-erbB2基因和EGFR可能参与了卵巢早衰中卵泡成熟障碍及卵泡凋亡的调控。
목적：탐토란소조쇠대서란소중원암기인c-erbB2화EGFR적표체。방법20지유동정주기적자성대서수궤분위공백조화실험조。실험조대서구복뢰공등다대편현부액,안조50mg/Kg.d，공14d,조POF모형；공백조대서동제량생리염수관위，1차/d，공14d。급약결속후24h,공백조화실험조대서취혈표본후처사，류취란소。류취적란소제절편후행HE염색화면역조화。결과란소HE염색발현공백조란포생장활약,가견교다적성숙화차급란포,이실험조란소차급화성숙란포명현감소(P<0.01)，가견교다폐쇄란포(P<0.01)，란포적과립세포층차명현감소；면역조화발현공백조ErbB2단백화EGFR단백양성신호명현，주요위우란모세포포막상，위심종색염색과립，실험조칙양성표체교소。결론 c-erbB2기인화EGFR가능삼여료란소조쇠중란포성숙장애급란포조망적조공。
Objective To explore the expressions of proto-oncogene c-erbB2 and EGFR in rats with premature ovarian fail-ure. Methods Twenty female estrus rats were randomly divided into blank group and experimental group. The rats in experimen-tal group were treated with pouring Tripterygium wilfordii polyglycoside (TWP) suspension liquid (50mg/kg·d) into stomach for 14 days;while the rats in blank group were treated with pouring normal saline of the same volume into stomach for 14 days. After the models were made, ovarian sections in the two groups were taken to assess the expression of proto-oncogene c-erbB2 and EGFR in ovarians and to observe the ovarian histomorphological changes. Results The numbers of mature and secondary follicles in ex-perimental groups were significantly reduced(P<0.01),whereas the numbers of atresia follicles were markedly increased as compared with that of the controls (P<0.01). The expression of ErbB2 and EGFR protein in the blank group was mainly on the cell membrane of oocytes, but their expression levels in the experimental group were much lower than that in different stages of the follicles. Conclusion EGFR and c-erbB2 might be involved in the maturation and apoptosis of ovarian follicles.