CAJ | 학술논문

比较了不同消毒剂和处理方式对“龙纹”豇豆种子的消毒效果及无菌生长的效应。结果表明：先消毒再浸种的效果较好，其中0.1%的升汞消毒8 min的效果最佳（污染率为11%，萌发率为77%）。消毒后将种子接种在附加有300 mg · L-1的头孢的培养基中，能有效减少培养过程中的污染率（7%），同时不影响无菌幼苗的生长，10 d后85%无菌苗株高可达3 cm以上。
비교료불동소독제화처리방식대“룡문”강두충자적소독효과급무균생장적효응。결과표명：선소독재침충적효과교호，기중0.1%적승홍소독8 min적효과최가（오염솔위11%，맹발솔위77%）。소독후장충자접충재부가유300 mg · L-1적두포적배양기중，능유효감소배양과정중적오염솔（7%），동시불영향무균유묘적생장，10 d후85%무균묘주고가체3 cm이상。
The effects of different seed disinfection methods on Longwen cultivar cowpea seeds were investigated. Optimum results were achieved by surface sterilizing the seeds with 0. 1% mercury bichloride solution for 8 minutes (contamination rate 11%,germination rate 77%). The contamination rate can be efficiently decreased by adding 300 mg · L-1 cefotaxime sodium to the medium after surface sterilization(7%). This method does not affect the normal growth of plants,85% of plants reach the height of 3cm after 10 days.