CAJ | 학술논문

从武汉豇豆种植基地的豇豆花苞中筛选出花内生菌（命名为wjhb），其在豇豆花苞中大量存在，在花瓣中为优势内生菌。染色鉴定为革兰氏阴性杆菌，可以在16～43℃温度生长；生化试验显示，该菌生化特征符合不动杆菌特征；16SrDNA扩增产物测序后，经软件分析其16SrDNA序列为与不动杆菌序列相似度达98%。本试验分离得到的内生不动杆菌（wjhb），有望成为豇豆基因工程生防菌的理想宿主菌株。
종무한강두충식기지적강두화포중사선출화내생균（명명위wjhb），기재강두화포중대량존재，재화판중위우세내생균。염색감정위혁란씨음성간균，가이재16～43℃온도생장；생화시험현시，해균생화특정부합불동간균특정；16SrDNA확증산물측서후，경연건분석기16SrDNA서렬위여불동간균서렬상사도체98%。본시험분리득도적내생불동간균（wjhb），유망성위강두기인공정생방균적이상숙주균주。
The entophyte bacteria(wjhb)was isolated from the cowpea flowers in Wuhan ,which was the dominant strain in the cowpea florescence. The proper temperature range for the entophyte bacteria culture was 16~43℃and the Gram staining result was negative. The biochemical experiment result showed the entophyte bacteria belonged to Acinetobacter sp. and the biochemical character was up to the Acinetobacter sp. The 16SrDNA sequence PCR results showed the similarity of 16SrDNA sequence with Acinetobacter sp. reached 98%. The entophyte Acinetobacter sp.(wjhb)in this paper was an ideal candidate of the host for cowpea engineered bio-control bacteria.