CAJ | 학술논문

以紫苏叶为外植体，采用单因素和正交设计试验研究了植物激素对紫苏叶愈伤组织诱导和黄酮积累的影响。结果表明：4种激素对紫苏叶愈伤组织诱导的影响依次为2，4-D>6-BA>NAA>KT；对愈伤组织积累黄酮的影响依次为2.4-D>6-BA>KT>NAA；紫苏叶愈伤组织培养的最佳激素组合为6-BA 2.0 mg/L+2，4-D 0.5 mg/L+KT 0.6 mg/L+NAA 0.8 mg/L；经愈伤组织培养获得的黄酮含量最高达到3.925%，远高于自然界紫苏叶黄酮含量。上述试验结果说明利用组培技术获得紫苏黄酮有可行性。
이자소협위외식체，채용단인소화정교설계시험연구료식물격소대자소협유상조직유도화황동적루적영향。결과표명：4충격소대자소협유상조직유도적영향의차위2，4-D>6-BA>NAA>KT；대유상조직적루황동적영향의차위2.4-D>6-BA>KT>NAA；자소협유상조직배양적최가격소조합위6-BA 2.0 mg/L+2，4-D 0.5 mg/L+KT 0.6 mg/L+NAA 0.8 mg/L；경유상조직배양획득적황동함량최고체도3.925%，원고우자연계자소협황동함량。상술시험결과설명이용조배기술획득자소황동유가행성。
In this study ,with the leaves of Perilla frutescen were used as explant ,effect of different concentrations of the hormone on inducing callus and formating flavonoid in Perilla frutescen leaves were studied by single factor and orthogonal test. It was found that the effect order of four kind of hormones to callus induction was 2,4-D>6-BA>NAA>KT and the order to flavonoid formation of the optimum medium for callus induction was MS+6-BA 2. 0 mg/L+2 ,4-D 0. 5 mg/L+KT 0. 6 mg/L+NAA 0. 8 mg/L by orthogonal design ,under the optimum conditions ,the highest indution rate was 83. 3%after 25 days. Furthemore ,the flavonoid formation of callus achieved at 3. 925%,it is much higher than the natural Perilla frutescen leaves ,the experimental results indicate the feasibility of flavonoid formation with tissue engineering.