CAJ | 학술논문

为减缓长期继代蝴蝶兰类原球茎增殖、萌发能力降低，在培养基中添加抗氧化剂继代类原球茎24个月，观测类原球茎增殖能力、成活率及萌发率，并分析其生理学指标。结果表明：100 mg？L 半胱氨酸与8．0 g？L甘露醇组合为适宜抗氧化剂浓度；在培养第24个月，处理组每瓶鲜重较对照组显著提高，萌发率极显著提高；培养期间对照组和处理组类原球茎的总抗氧化能力、超氧化物歧化酶、过氧化物酶活性均有不同程度的下降，处理组总抗氧化能力、超氧化物歧化酶、过氧化氢酶活性较对照极显著提高（主要在第16个月），对照组与处理组叶绿素水平均变化不明显。
위감완장기계대호접란류원구경증식、맹발능력강저，재배양기중첨가항양화제계대류원구경24개월，관측류원구경증식능력、성활솔급맹발솔，병분석기생이학지표。결과표명：100 mg？L 반광안산여8．0 g？L감로순조합위괄의항양화제농도；재배양제24개월，처리조매병선중교대조조현저제고，맹발솔겁현저제고；배양기간대조조화처리조류원구경적총항양화능력、초양화물기화매、과양화물매활성균유불동정도적하강，처리조총항양화능력、초양화물기화매、과양화경매활성교대조겁현저제고（주요재제16개월），대조조여처리조협록소수평균변화불명현。
To slow down the decline of proliferation and germination capabilities of Phalaenopsis protocorm-like bodies (PLBs)in long-term subculture,antioxidants were added to the media,the PLBs’proliferation capa-bility and survival and germination rates were observed in 24-month subculture,and their physiological indexes were also analyzed.The results showed that a suitable antioxidant combination was 100 mg?Lcysteine and 8.0 g?L mannitol;The treatment group’s fresh weight per culture bottle in the 24th month was significantly greater than that of the control,and the PLBs’germination rate was improved very significantly;During PLBs subculture all the total antioxidant capacity and SOD and POD activities decreased to different extents in both treatment group and control group,but all the total antioxidant capacity and SOD and CAT activities in the treatment group were very significantly higher than those in the control group (mainly in the 16th month),yet their chlorophyll con-tents in both groups did not very obviously.