CAJ | 학술논문

采用廉价的聚乙二醇(PEG)/酒石酸钾钠建立双水相体系,从干螺旋藻中分离纯化藻蓝蛋白C-PC。以纯度(目的蛋白在620 nm处的特征吸光值和总蛋白在280 nm处吸光值的比值)为指标,优化体系参数。研究结果表明,体系pH 8.06,系线长为21.65%、体积比为0.22的PEG1000和酒石酸钾钠双水相体系分离纯化效果最好,纯度由0.42提高到1.27,高于食品级纯度0.7,纯化因子3.04,回收率85.11%。硫酸铵盐析提高粗提液纯度后,二次双水相纯度达到3.28,高于药品级3.0。得到的 C-PC具有较高的生物活性。
채용렴개적취을이순(PEG)/주석산갑납건립쌍수상체계,종간라선조중분리순화조람단백C-PC。이순도(목적단백재620 nm처적특정흡광치화총단백재280 nm처흡광치적비치)위지표,우화체계삼수。연구결과표명,체계pH 8.06,계선장위21.65%、체적비위0.22적PEG1000화주석산갑납쌍수상체계분리순화효과최호,순도유0.42제고도1.27,고우식품급순도0.7,순화인자3.04,회수솔85.11%。류산안염석제고조제액순도후,이차쌍수상순도체도3.28,고우약품급3.0。득도적 C-PC구유교고적생물활성。
This paper used commercial aqueous two-phase system (ATPS) to efficiently extract and purify C-PC from the dryspirulina platensis. The purity of C-PC was defined as the relation between of 620 and 280 nm ab-sorbance, which was as the target to optimize the system parameters. The optimal conditions were proved in poly-ethylene glycol (PEG) 1000 and potassium sodium tartrate, system pH of 8.06, the tie-line length (TLL) of 21.65% (w/w) and the volume ratio (Vr) of 0.22 to increase the purity from the initial purity of 0.42 to 1.27 after the first extraction. The purity was over the food grade 0.7 and the purification factor was 3.04. The recovery yield was 85.11%. The purity was achieved up 3.28 after the saturation of ammonium sulfate in the second ATPS extraction. It was more than the pharmaceutical grade 3.0.