CAJ | 학술논문

采用2216E、R2A、M1培养基，首次从俄罗斯库页岛潮间带沉积物样品中分离到82株可培养海洋细菌，经查重并菌后选取其中45株具有代表性的菌株进行了16S rRNA基因序列分析和多样性研究。结果发现，它们共分布于变形细菌门(Proteobacteria)、放线菌门(Actinobacteria )、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)4个类群(分别占45株代表菌株的42.2%、20.0%、20.0%和17.8%)，代表着6个纲、27个属、44个种；同时发现其中有8个潜在新种和1个潜在新属。本研究结果表明，俄罗斯库页岛潮间带沉积物可培养细菌的系统发育多样性非常高，大部分菌株与分离自日本和韩国近海的细菌物种在系统发育关系上高度同源，但也存在不少潜在的新物种。
채용2216E、R2A、M1배양기，수차종아라사고혈도조간대침적물양품중분리도82주가배양해양세균，경사중병균후선취기중45주구유대표성적균주진행료16S rRNA기인서렬분석화다양성연구。결과발현，타문공분포우변형세균문(Proteobacteria)、방선균문(Actinobacteria )、의간균문(Bacteroidetes)화후벽균문(Firmicutes)4개류군(분별점45주대표균주적42.2%、20.0%、20.0%화17.8%)，대표착6개강、27개속、44개충；동시발현기중유8개잠재신충화1개잠재신속。본연구결과표명，아라사고혈도조간대침적물가배양세균적계통발육다양성비상고，대부분균주여분리자일본화한국근해적세균물충재계통발육관계상고도동원，단야존재불소잠재적신물충。
Four intertidal sediment samples collected from Russian Sakhalin Island were treated with plate dilution method and the culturable marine bacteria were isolated. The diversity of marine bacteria was examined based on 16S rRNA gene sequence analysis. A total of 45 representative strains were selected from 82 isolates, and were further sequenced and the phylogenetic analysis was carried out. The results showed that the 45 representative strains were composed of 4 major lineages including Proteobacteria (42.2%), Actinobacteria (20%), Bacteroidetes (20%) and Firmicutes (17.8%), and belonged to 6 classes, 27 genera and 44 species. Meanwhile, 8 strains were identified as potential novel species and 1 strain was identified as potential novel genus. This study indicated that there was high bacterial diversity in intertidal sediment samples of Russian Sakhalin Island and most of the strains were very close to the bacteria species isolated from coastal area of Japan and South Korea. In addition, many po-tential new species were found.