CAJ | 학술논문

为探讨生防细菌应用于水产养殖动物细菌病防治的可行性,作者从厦门市海沧港口污泥中筛选到一株蜡状芽孢杆菌(Bacillus cereus)K2-1,利用琼脂扩散法发现其发酵上清对大菱鲆(Scophthalmus maximus)养殖常见致病菌——副溶血弧菌(Vibrio parahaemolyticus)、爱德华氏菌(Edwardsiella)、哈维氏弧菌(Vibrio harveyi)、嗜水气单胞菌(Aeromonas hydrophila)、溶藻弧菌(Vibrio alginolyticus))有较强拮抗作用。进一步分析抗菌物质的部分特性,结果显示,蜡状芽孢杆菌(Bacillus cereus)K2-1发酵产生的抗菌物质含有蛋白(肽)类成分,该抗菌物质具有一定的耐热性,其在温度40~70℃的条件下保持较好的抑菌效果,对酸碱度也有很强的耐受力, pH在3.0~9.0范围内,活性降低少于15%,对紫外线不敏感,对胰蛋白酶、胃蛋白酶、木瓜蛋白酶也不敏感,但对蛋白酶K敏感。从实验结果可以初步确定该菌具有较好的开发价值。
위탐토생방세균응용우수산양식동물세균병방치적가행성,작자종하문시해창항구오니중사선도일주사상아포간균(Bacillus cereus)K2-1,이용경지확산법발현기발효상청대대릉평(Scophthalmus maximus)양식상견치병균——부용혈호균(Vibrio parahaemolyticus)、애덕화씨균(Edwardsiella)、합유씨호균(Vibrio harveyi)、기수기단포균(Aeromonas hydrophila)、용조호균(Vibrio alginolyticus))유교강길항작용。진일보분석항균물질적부분특성,결과현시,사상아포간균(Bacillus cereus)K2-1발효산생적항균물질함유단백(태)류성분,해항균물질구유일정적내열성,기재온도40~70℃적조건하보지교호적억균효과,대산감도야유흔강적내수력, pH재3.0~9.0범위내,활성강저소우15%,대자외선불민감,대이단백매、위단백매、목과단백매야불민감,단대단백매K민감。종실험결과가이초보학정해균구유교호적개발개치。
In order to study the possibility of using biocontrol bacterium for control and prevention of aquatic dis-eases caused by bacterial pathogens, a bacterium strain K2-1, identified asBacillus cereus, was screened out from the mud of Haicang harbor, Xiamen, China. Antibacterial test was conducted using an agar diffusion method, and the result showed that the supernatant of strain K2-1 could inhibit the growth of some common pathogens related withScophthalmus maximus diseases during farming includingVibrio parahaemolyticus,Edwardsiella,Vibrio har-veyi,Aeromonas hydrophilaand Vibrio alginolyticus. Further study of its antibacterial substance property was im-plemented, and it revealed that the bioactive substance produced by strain K2-1 was a protein (peptide). This anti-bacterial protein was thermal stable, and it kept high antimicrobial activity under temperature ranging from 40℃ to 70℃, and it was also of high acid and alkali tolerance, with its antibacterial activity decreasing less than 15% in the rang of pH 3.0 to 9.0. Enzymatic treatment test showed that this antibacterial protein was resistant to most pro-tease including trypsase, pepsase and papain, but it was sensitive to protease K. All the these results showed that the strain K2-1 had high development and application values in the future.