上海农业学报
上海農業學報
상해농업학보
Acta Agriculturae Shanghai
2015年
5期
74-78
,共5页
宋献策%张建军%顾巧英%王奕
宋獻策%張建軍%顧巧英%王奕
송헌책%장건군%고교영%왕혁
非洲紫罗兰%光照强度%蔗糖浓度%离体培养
非洲紫囉蘭%光照彊度%蔗糖濃度%離體培養
비주자라란%광조강도%자당농도%리체배양
Saintpaulia ionantha%Light intensity%Sucrose concentration%In vitro culture
以试管苗为材料，研究了光（0—3340 lx）和碳源（0％—3％的蔗糖）对非洲紫罗兰离体培养的影响。结果表明：非洲紫罗兰可在没有外加碳源的有光照条件下存活、生长和分化，其繁殖率和生长量与光照强度正相关，但离体培养效果不及添加蔗糖的处理。弱光（379—405 lx）与3％的蔗糖组合最有利于叶片不定芽诱导，强光（3110—3340 lx）与3％的蔗糖组合更有利于试管苗不定芽的扩繁，强光（3110—3340 lx）和1％的蔗糖组合较适宜非洲紫罗兰壮苗与生根培养。
以試管苗為材料，研究瞭光（0—3340 lx）和碳源（0％—3％的蔗糖）對非洲紫囉蘭離體培養的影響。結果錶明：非洲紫囉蘭可在沒有外加碳源的有光照條件下存活、生長和分化，其繁殖率和生長量與光照彊度正相關，但離體培養效果不及添加蔗糖的處理。弱光（379—405 lx）與3％的蔗糖組閤最有利于葉片不定芽誘導，彊光（3110—3340 lx）與3％的蔗糖組閤更有利于試管苗不定芽的擴繁，彊光（3110—3340 lx）和1％的蔗糖組閤較適宜非洲紫囉蘭壯苗與生根培養。
이시관묘위재료，연구료광（0—3340 lx）화탄원（0％—3％적자당）대비주자라란리체배양적영향。결과표명：비주자라란가재몰유외가탄원적유광조조건하존활、생장화분화，기번식솔화생장량여광조강도정상관，단리체배양효과불급첨가자당적처리。약광（379—405 lx）여3％적자당조합최유리우협편불정아유도，강광（3110—3340 lx）여3％적자당조합경유리우시관묘불정아적확번，강광（3110—3340 lx）화1％적자당조합교괄의비주자라란장묘여생근배양。
Effects of light (0—3 340 lx)and carbon (0%—3% sucrose)on in vitro culture of Saintpaulia ionantha plantlets were studied taking the tube seedling as material.The results showed that Saintpaulia ionantha could survive,grow and differentiate in the light condition without carbon sources.The reproduction rate and growth were positively correlated with the light intensity,but the effect of in vitro culture condition without sucrose was less than that of sucrose treatment.Lower Light (379—405 lx)and 3% sucrose treatment on leaves were conducive to adventitious buds induction.Strong light (3 110—3 340 lx)and 3% sucrose treatment were more conducive to the propagation of adventitious buds.Strong light (3 110—3 340 lx)and 1% sucrose treatment were appropriate for seedling and rooting culture of Saintpaulia ionantha.
