CAJ | 학술논문

制备了空心碳球—离子液体1－丁基－3－甲基咪唑磷钨酸盐（HCS －[Bmim]3 PW12 O40）修饰玻碳（GC）电极，采用交联法将乙酰胆碱酯酶（AChE）固定在修饰电极表面，获得 AChE ／HCS －[Bmim]3 PW12 O40／GC 传感器。在最优条件下，用该传感器对有机磷农药久效磷、克百威标准品分别进行检测，久效磷的线性范围为8．00×10－10～8．00×10－6 g／L，克百威的线性范围是1．00×10－10～1．00×10－7 g／L。按抑制率10％计算，久效磷和克百威的检出限分别为7．58×10－11 g／L 和6．76×10－11 g／L。所制备的 AChE ／HCS －[Bmim]3 PW12 O40／GC 传感器灵敏度高和稳定性好，为有机磷农药的分析提供了一种有前景的工具。
제비료공심탄구—리자액체1－정기－3－갑기미서린오산염（HCS －[Bmim]3 PW12 O40）수식파탄（GC）전겁，채용교련법장을선담감지매（AChE）고정재수식전겁표면，획득 AChE ／HCS －[Bmim]3 PW12 O40／GC 전감기。재최우조건하，용해전감기대유궤린농약구효린、극백위표준품분별진행검측，구효린적선성범위위8．00×10－10～8．00×10－6 g／L，극백위적선성범위시1．00×10－10～1．00×10－7 g／L。안억제솔10％계산，구효린화극백위적검출한분별위7．58×10－11 g／L 화6．76×10－11 g／L。소제비적 AChE ／HCS －[Bmim]3 PW12 O40／GC 전감기령민도고화은정성호，위유궤린농약적분석제공료일충유전경적공구。
Hollow carbon spheres - ionic liquid 1 - butyl -3 - methyl imidazole phosphotungstate (HCS -[Bmim]3 PW12 O40 )was prepared to modify glassy carbon (GC)electrode.Acetylcholinesterase (AChE)was immobilized on the electrode surface by cross -linking reaction to obtain AChE /HCS -[Bmim]3 PW12 O40 /GC sensor.Under the optimal condition,the organophosphorus pesticides,monocro-tophos and carbofuran,were detected by the biosensor.The linear range of monocrotophos was 8.00 × 10 -10 ~8.00 ×10 -6 g/L,and that of carbofuran 1.00 ×10 -10 ~1.00 ×10 -7 g/L,with the detection limits of 7.58 ×10 -11 g/L and 6.76 ×10 -11 g/L,respectively,calculated by inhibition rate 10%.The biosensor exhibited high sensitivity and acceptable stability,and could be a promising tool for analysis of organophosphorus pesticides.