粮油食品科技
糧油食品科技
량유식품과기
Science and Technology of Cereals, Oils and Foods
2015年
5期
97-100
,共4页
芦荟%提取物%α-葡萄糖苷酶%抑制活性
蘆薈%提取物%α-葡萄糖苷酶%抑製活性
호회%제취물%α-포도당감매%억제활성
aloe%extract%α-glucosidase%inhibitory activity
为探讨芦荟醇提物对α－葡萄糖苷酶的抑制活性，从5种芦荟中筛选出对α－葡萄糖苷酶抑制效果最好的品种，以期从该芦荟中获得降低餐后血糖的功能成分。采用α－葡萄糖苷酶抑制剂体外筛选模型，通过抑制率高低确定抑制作用最好的芦荟品种，以酶浓度—反应速度及 Lin-eweaver －Burk 作图法确定酶抑制类型。结果发现，5种芦荟中木立芦荟醇提物抑制率为87．95％，效果最好；芦荟提取物是α－葡萄糖苷酶的非竞争性抑制剂，且呈剂量依赖性关系，其 Km 为0．442 mmol ／L；酶抑制动力学进程曲线表明其反应初速度的时间为18 min。以上结果提示，芦荟中含有活性较高的α－葡萄糖苷酶抑制剂，可开发用于糖尿病及其并发症的治疗与预防。
為探討蘆薈醇提物對α－葡萄糖苷酶的抑製活性，從5種蘆薈中篩選齣對α－葡萄糖苷酶抑製效果最好的品種，以期從該蘆薈中穫得降低餐後血糖的功能成分。採用α－葡萄糖苷酶抑製劑體外篩選模型，通過抑製率高低確定抑製作用最好的蘆薈品種，以酶濃度—反應速度及 Lin-eweaver －Burk 作圖法確定酶抑製類型。結果髮現，5種蘆薈中木立蘆薈醇提物抑製率為87．95％，效果最好；蘆薈提取物是α－葡萄糖苷酶的非競爭性抑製劑，且呈劑量依賴性關繫，其 Km 為0．442 mmol ／L；酶抑製動力學進程麯線錶明其反應初速度的時間為18 min。以上結果提示，蘆薈中含有活性較高的α－葡萄糖苷酶抑製劑，可開髮用于糖尿病及其併髮癥的治療與預防。
위탐토호회순제물대α－포도당감매적억제활성，종5충호회중사선출대α－포도당감매억제효과최호적품충，이기종해호회중획득강저찬후혈당적공능성분。채용α－포도당감매억제제체외사선모형，통과억제솔고저학정억제작용최호적호회품충，이매농도—반응속도급 Lin-eweaver －Burk 작도법학정매억제류형。결과발현，5충호회중목립호회순제물억제솔위87．95％，효과최호；호회제취물시α－포도당감매적비경쟁성억제제，차정제량의뢰성관계，기 Km 위0．442 mmol ／L；매억제동역학진정곡선표명기반응초속도적시간위18 min。이상결과제시，호회중함유활성교고적α－포도당감매억제제，가개발용우당뇨병급기병발증적치료여예방。
The inhibitory effects of aloe extracts on α-glucosidase activity were investigated.The aloe with the best inhibitory effects on α-glucosidase was screened from 5 species,in order to obtain the functional composition of reducing the postprandial blood glucose.The best variety of aloe was determined according to the inhibition rate by α-glucosidase inhibitor screen model in vitro;and the type of enzyme inhibition was decided by enzyme concentration -response speed and L -B graphing method.Results showed that the ethanol extract of aloe arborescens miller showing the best effects had the highest inhibi-tion rate of 87.95%;aloe extract was noncompetitive inhibitor of α-glucosidase and showed dose -de-pendent relationship.The Km value of aloe extract was 0.442 mmol /L.The enzyme inhibition kinetics process curve showed the time of initial velocity of reaction was 18 min.It suggested that aloe containedα-glucosidase inhibitors with high effects,and could be developed for the treatment and prevention of diabetes and its complications.
